Late Paleocene–early Eocene Arctic Ocean sea surface temperatures: reassessing biomarker paleothermometry at Lomonosov Ridge
- 1Department of Earth Sciences, Faculty of Geosciences, Utrecht University, Princetonlaan 8a, 3584 CB Utrecht, the Netherlands
- 2Organic Geochemistry Unit, School of Chemistry, School of Earth Sciences, University of Bristol, Bristol, UK
- 3NIOZ Royal Institute for Sea Research, Department of Microbiology and Biogeochemistry, P.O. Box 59, 1790 AB Den Burg, the Netherlands
- apresent address: School of Ocean and Earth Science, National Oceanography Centre Southampton, University of Southampton, Southampton, UK
Correspondence: Appy Sluijs (firstname.lastname@example.org)
A series of papers published shortly after the Integrated Ocean Drilling Program Arctic Coring Expedition (ACEX, 2004) on Lomonosov Ridge indicated remarkably high early Eocene sea surface temperatures (SSTs; ca. 23 to 27 ∘C) and land air temperatures (ca. 17 to 25 ∘C) based on the distribution of isoprenoid and branched glycerol dialkyl glycerol tetraether (isoGDGT and brGDGT) lipids, respectively. Here, we revisit these results using recent analytical developments – which have led to improved temperature calibrations and the discovery of new temperature-sensitive glycerol monoalkyl glycerol tetraethers (GMGTs) – and currently available proxy constraints.
The isoGDGT assemblages support temperature as the dominant variable controlling TEX86 values for most samples. However, contributions of isoGDGTs from land, which we characterize in detail, complicate TEX86 paleothermometry in the late Paleocene and part of the interval between the Paleocene–Eocene Thermal Maximum (PETM; ∼ 56 Ma) and the Eocene Thermal Maximum 2 (ETM2; ∼ 54 Ma). Background early Eocene SSTs generally exceeded 20 ∘C, with peak warmth during the PETM (∼ 26 ∘C) and ETM2 (∼ 27 ∘C). We find abundant branched GMGTs, likely dominantly marine in origin, and their distribution responds to environmental change. Further modern work is required to test to what extent temperature and other environmental factors determine their distribution.
Published Arctic vegetation reconstructions indicate coldest-month mean continental air temperatures of 6–13 ∘C, which reinforces the question of whether TEX86-derived SSTs in the Paleogene Arctic are skewed towards the summer season. The exact meaning of TEX86 in the Paleogene Arctic thus remains a fundamental issue, and it is one that limits our assessment of the performance of fully coupled climate models under greenhouse conditions.
The Eocene epoch (56 to 34 million years ago; Ma) has long been characterized by warm climates. The earliest signs of a balmy Eocene Arctic region – fossil leaves of numerous plant species – were documented 150 years ago (Heer, 1869). Subsequent findings identified palms, baobab and mangroves, indicating the growth of temperate rainforests and year-round frost-free conditions in the Eocene Arctic region (Schweitzer, 1980; Greenwood and Wing, 1995; Suan et al., 2017; Willard et al., 2019). Fossils of animals, including varanid lizards, tortoises and alligators, also indicate warm Arctic climates (Dawson et al., 1976; Estes and Hutchinson, 1980). These earliest findings sparked interest in the climatological mechanisms allowing for such polar warmth about a century ago (Berry, 1922). Ever since, paleobotanists have focused on Arctic plant fossils and significantly refined their paleoclimatological interpretation towards estimates of precipitation as well as seasonal and mean annual temperature (e.g., Uhl et al., 2007; Greenwood et al., 2010; Eberle and Greenwood, 2012; Suan et al., 2017; Willard et al., 2019).
Novel insights in Paleogene Arctic paleoclimate research were made in the years following the Arctic Coring Expedition 302 (ACEX; Integrated Ocean Drilling Program – IODP, 2004; Fig. 1). This expedition recovered upper Paleocene and lower Eocene siliciclastic sediments deposited in a shallow marine environment, in Hole 4A (87∘52.00′ N; 136∘10.64′ E; 1288 m water depth), on the Lomonosov Ridge in the central Arctic Ocean (Backman et al., 2006). The succession was deposited at a paleolatitude of ∼ 78∘ N based on a geological reconstruction (Seton et al., 2012) projected using a paleomagnetic reference frame (Torsvik et al., 2012) (see http://paleolatitude.org/, model version 2.1; Van Hinsbergen et al., 2015). The sediments are devoid of biogenic calcium carbonate but rich in immature organic matter, including terrestrial and marine microfossil assemblages and molecular fossils (e.g., Pagani et al., 2006; Sluijs et al., 2006; Stein et al., 2006).
As the upper Paleocene and lower Eocene sediments of the ACEX core lack biogenic calcium carbonate and alkenones, sea surface temperature (SST) reconstructions are based on the biomarker-based paleothermometer TEX86. This proxy is based on membrane lipids (isoprenoid glycerol dibiphytanyl glycerol tetraethers; isoGDGTs) of Thaumarchaeota, which adapt the fluidity of their membrane according to the surrounding temperature by increasing the number of cyclopentane rings at higher temperatures (De Rosa et al., 1980; Wuchter et al., 2004; Schouten et al., 2013, and references therein). The proxy was introduced in 2002 by Schouten et al. (2002) and was calibrated to mean annual SST using modern marine surface sediments.
Initial papers suggested that Arctic SST increased significantly during two episodes of transient global warming. Maximum values of ∼ 23 and ∼ 27 ∘C occurred during the Paleocene–Eocene Thermal Maximum (PETM-56 Myr ago; Sluijs et al., 2006) and the Eocene Thermal Maximum 2 (ETM2-54 Myr ago; Sluijs et al., 2009), respectively. Lower SSTs, generally exceeding 20 ∘C, characterized the remainder of the early Eocene (Sluijs et al., 2008b). Such temperatures were immediately recognized to be remarkably high and could not be explained using fully coupled climate model simulations (Sluijs et al., 2006). Even the current-generation of IPCC-class (IPCC: Intergovernmental Panel on Climate Change) models are unable to match early Eocene Arctic mean annual SSTs, although reconstructions of tropical and midlatitude SSTs and deep ocean temperatures are consistent with some newer simulations (Frieling et al., 2017; Cramwinckel et al., 2018; Evans et al., 2018; Zhu et al., 2019).
Since the publication of the ACEX SST records, constraints on the applicability of the TEX86 proxy have tremendously improved (see review by Schouten et al., 2013, and subsequent work by Taylor et al., 2013; Elling et al., 2014; Qin et al., 2014; Elling et al., 2015; Kim et al., 2015; Qin et al., 2015; Hurley et al., 2016; Zhang et al., 2016). This work has delivered new constraints on the ecology of Thaumarchaeota, the dominant depth at which they reside in the ocean and the depth from which their isoGDGTs are exported towards the seafloor. It also identified potential confounding factors such as variation in dominant isoGDGT export depth (e.g., Taylor et al., 2013; Kim et al., 2015), the input of non-thaumarchaeotal-derived isoGDGTs (e.g., Weijers et al., 2011; Zhang et al., 2011), growth phase (Elling et al., 2014), and environmental ammonium and oxygen concentrations (Qin et al., 2015; Hurley et al., 2016). Moreover, several indicators to detect such anomalies have been developed. Improvements in the chromatography method used for GDGT analysis now allow for better separation of previously co-eluting compounds, leading to enhanced analytical precision and sensitivity (Hopmans et al., 2016). Finally, recent work has described new GDGTs from oceans and sediments, notably branched glycerol monoalkyl glycerol tetraethers (brGMGTs or “H-shaped” brGDGTs) (e.g., Schouten et al., 2008; Liu et al., 2012), characterized by a covalent carbon–carbon bond that links the two alkyl chains. Their presence and distribution in peats and lake sediments have been linked to land air temperatures (LAT) (e.g., Naafs et al., 2018a; Baxter et al., 2019). However, these compounds have not yet been reported from ancient marine sediments.
Considering these developments and the paleoclimatological importance of the ACEX dataset, we reanalyzed the original lipid extracts for the PETM, ETM2 and the interval spanning these events (Sluijs et al., 2006; Sluijs et al., 2009) according to the latest chromatography protocols. We also compile published and generate new GDGT data from modern and Paleogene terrestrial deposits and use these to better assess the potential confounding influence of isoGDGTs from terrestrial sources, which was already recognized as a potential problem in the early work (Sluijs et al., 2006).
2.1 TEX86 and its calibration to SST
TEX86 is based on the relative abundance of four different GDGTs (Fig. 2), following (Schouten et al., 2002)
where a higher relative abundance of cyclopentane moieties implies higher SSTs.
A number of models are used to calibrate TEX86 to SST (Schouten et al., 2002; Schouten et al., 2003; Schouten et al., 2007; Kim et al., 2008; Liu et al., 2009; Kim et al., 2010; Tierney and Tingley, 2014; O'Brien et al., 2017), all based on a modern ocean surface sediment database. The currently available culture and mesocosm experiments as well as surface sediment data suggest that the relation between SST and TEX86 is close to linear for a large portion of the modern ocean (Kim et al., 2010; Ho et al., 2014; Tierney and Tingley, 2014; O'Brien et al., 2017). In polar regions, the TEX86 response to temperature diminishes (e.g., Kim et al., 2010; Tierney and Tingley, 2014). The response of TEX86 to SST at the high-temperature end remains the subject of discussion (e.g., Cramwinckel et al., 2018; Hollis et al., 2019). Several authors prefer a linear relation (e.g., Tierney and Tingley, 2014; O'Brien et al., 2017). However, physiological considerations and multiple temperature-dependent GDGT indices might also imply a nonlinear relation at the high-temperature end, as can be observed at the high end of the modern ocean dataset and beyond the reach of the modern ocean in paleoclimate data (Cramwinckel et al., 2018). At higher temperatures, membrane adaptation may increasingly be established using isoGDGTs not included in the TEX86 ratio, leading to a diminished TEX86 response at very high temperatures (Cramwinckel et al., 2018). A nonlinear response has thus been proposed in other calibrations (Liu et al., 2009; Kim et al., 2010). The most recent nonlinear calibration, (Kim et al., 2010), represents an exponential relation between SST and TEX86 (Hollis et al., 2019). Unfortunately, is mathematically problematic and has systematic residuals in the modern ocean (Tierney and Tingley, 2014).
Tierney and Tingley (2014) introduced a spatially varying Bayesian method to convert TEX86 to SST and assume a linear relationship (BAYSPAR). BAYSPAR extracts TEX86 values from the modern core-top dataset that are similar to the measured TEX86 value from the geological sample based on a tolerance defined by the user, and it subsequently calculates regressions based on these core-top data. The uncertainty in SST reflects spatial differences in the correlation coefficient, intercept and error variance of the regression model.
Currently, it is generally encouraged to present results using both a linear and a nonlinear function (Hollis et al., 2019). The assumption of a linear or nonlinear relation between SST and TEX86 leads to very different SST reconstructions for geological samples when TEX86 values are > 0.70 (Kim et al., 2010; Tierney and Tingley, 2014; Frieling et al., 2017; O'Brien et al., 2017; Cramwinckel et al., 2018; Hollis et al., 2019). However, TEX86 values for the early Eocene ACEX samples (0.5–0.7, Sluijs et al., 2006; Sluijs et al., 2008b; Sluijs et al., 2009) are below this value and well above most values observed in the polar regions (Kim et al., 2010; Tierney and Tingley, 2014; O'Brien et al., 2017), indicating that all calibrations will yield similar absolute SST values.
2.2 Caveats and confounding factors
Several confounding factors and caveats have been identified that could potentially bias TEX86 data relative to mean annual SST. These notably relate to additions of isoGDGTs that were not produced in the upper water column by Thaumarchaeota, seasonal biases and choices that are made in the calibration between SST and TEX86. Below we summarize methods that have been developed to assess if isoGDGT distributions might have been biased by confounding factors.
2.2.1 isoGDGTs of terrestrial origin
Previous work (Sluijs et al., 2006; Sluijs et al., 2008b; Sluijs et al., 2009) recognized that high contributions of terrestrially derived isoGDGTs could compromise the TEX86 signal for portions of the upper Paleocene to lower Eocene interval of the ACEX core. This contribution can be tracked using the branched and isoprenoid tetraether (BIT) index, a ratio of mostly soil-derived branched GDGTs (brGDGTs; Fig. 2) and crenarchaeol, which is dominantly marine-derived (Hopmans et al., 2004; Schouten et al., 2013; adjusted to include 6-methyl brGDGT isomers):
Most studies define a BIT value (typically 0.3 or 0.4) above which TEX86-derived SSTs are unreliable (e.g., Weijers et al., 2006). However, the threshold of 0.4 is conservative in some settings, and the impact of terrigenous GDGTs on reconstructed SST will depend on the nature and temperature of the source catchment (Inglis et al., 2015). In addition, a cutoff value based on BIT values is difficult given the relatively large differences in BIT between labs, which originate from methodological differences (Schouten et al., 2009). A strong linear relationship between BIT and TEX86 values is often taken as an indication of a bias in TEX86 through land-derived isoGDGTs to the marine TEX86 signature (e.g., Douglas et al., 2014). An earlier study used a somewhat subjective threshold of 0.3 for an interval spanning ETM2 in the ACEX core (Sluijs et al., 2009).
2.2.2 isoGDGTs of deepwater origin
Thaumarchaeota, the source of most isoGDGTs in marine waters (Zeng et al., 2019; Besseling et al., 2020), are ammonium oxidizers (Könneke et al., 2005; Wuchter et al., 2006a), making them independent of light. Although they occur throughout the water column, maximum abundances occur at depths < 200 m, generally around NO2 maxima (e.g., Karner et al., 2001; Pitcher et al., 2011a). In most oceans, sedimentary GDGTs dominantly derive from the upper few hundred meters based on analyses of suspended particular organic matter and sediment traps (Wuchter et al., 2005; Wuchter et al., 2006b; Yamamoto et al., 2012; Richey and Tierney, 2016). A deeper contribution has also been inferred based on 14C analysis (Shah et al., 2008), implying possible contributions of isoGDGTs from the thermocline. Moreover, contributions of isoGDGTs produced in the deep sea have regionally been identified (e.g., Kim et al., 2015). Taylor et al. (2013) also found that deep-dwelling (> 1000 m) archaea might contribute to the sedimentary isoGDGT assemblage. They indicate that such deep contributions can be tracked using the GDGT-2 ∕ GDGT-3 ratio; high values of > 5 indicate contributions of archaea living deeper in the water column. Given that upper Paleocene and lower Eocene ACEX sediments were deposited in a shallow shelf environment (Sluijs et al., 2008b), a significant contribution of deep ocean archaeal lipids is not expected.
2.2.3 isoGDGTs of methanotrophic and methanogenic archaea
Contributions of isoGDGTs to the sedimentary pool might also derive from anaerobic methanotrophs and/or methanogens. Several indices have been developed to track such contributions based on relatively high contributions of particular isoGDGTs of these groups of archaea. The methane index (MI) was developed to detect the relative contribution of anaerobic methanotrophic Euryarchaeota, notably represented by GDGT-1, GDGT-2 and GDGT-3 (Pancost et al., 2001; Zhang et al., 2011), and is therefore defined as
MI values greater than 0.5 indicate a significant contribution of anaerobic methanotrophy. Such values may yield unreliable TEX86 values. Another tracer for contributions of anaerobic methanotrophic archaea is the analogous GDGT-2 ∕ crenarchaeol ratio (Weijers et al., 2011).
Methanogenic archaea can synthesize GDGT-0 and smaller quantities of GDGT-1, GDGT-2 and GDGT-3. The ratio GDGT-0 ∕ crenarchaeol is indicative of contributions of methanogenic archaea to the isoGDGT pool (Blaga et al., 2009); values > 2 indicate a substantial contribution of methanogenic archaea. Up to now, high index values have often been observed near methane seeps or anoxic basins (e.g., Jaeschke et al., 2012) but rarely in open marine waters in the modern domain and paleodomains (Inglis et al., 2015; Zhang et al., 2016). Given the reducing conditions in the sediment and water column at the study site across the late Paleocene and early Eocene (Sluijs et al., 2006; Stein et al., 2006; Sluijs et al., 2008b; März et al., 2010), an influence of methane cycling might be expected.
2.2.4 isoGDGTs of the “Red Sea type”
Sedimentary isoGDGT distributions from the Red Sea are anomalous to other marine settings and are characterized by the low abundance of GDGT-0 and the high abundance of the crenarchaeol isomer. Presumably, this is due to an endemic thaumarchaeotal assemblage. The Red Sea isoGDGT distribution yields a different relationship between SST and TEX86 (Trommer et al., 2009; Kim et al., 2015). Inglis et al. (2015) attempted to quantify a “Red Sea type” GDGT distribution in geological samples using the following index:
However, as noted by Inglis et al. (2015) this ratio is also strongly SST-dependent such that the Red Sea type GDGT assemblage cannot be discerned from GDGT distributions that occur at high temperatures in normal open marine settings.
2.2.5 Seasonal bias
TEX86 is calibrated to mean annual SST. However, particularly in midlatitude and high-latitude areas where production and export production are highly seasonal, the sedimentary GDGT distribution might not represent annual mean conditions (Wuchter et al., 2006b; Pitcher et al., 2011b; Mollenhauer et al., 2015; Richey and Tierney, 2016; Park et al., 2019). This issue should partly be reflected in the calibration uncertainty of the modern ocean database (several degrees Celsius, depending on the calibration and method; see section 2.7). Sluijs et al. (2006, 2008b, 2009) originally argued that the TEX86 results from the ACEX core could be biased towards summer temperature because the export of organic matter from the surface ocean towards the sediment likely peaked during the season of highest production, i.e., the summer. However, we also note that the TEX86–temperature relationship is not improved when using seasonal mean ocean temperatures (Kim et al., 2010; Tierney and Tingley, 2014) and modern observations indicate homogenization of the seasonal cycle at depth (Wuchter et al., 2006b; Yamamoto et al., 2012; Richey and Tierney, 2016), implying that seasonality has a relatively limited effect on modern sedimentary TEX86 values.
2.2.6 Additional isoGDGT-based temperature indicators
The underlying mechanism of TEX86 is that isoGDGTs produced at higher SSTs contain more rings than those produced at low SSTs. Although the combination of compounds included in TEX86 seems to yield the strongest relation with temperature in the modern ocean (Kim et al., 2010), it implies that isoGDGT ratios other than TEX86 also provide insights into SST. One alternative temperature-sensitive isoGDGT index is the ring index (RI), which represents the weighed number of cyclopentane rings of isoGDGTs 0–3, crenarchaeol and the crenarchaeol isomer (Zhang et al., 2016), defined as
Note that the abundance of GDGT-0 is important for determining the percentage of the other GDGTs in the total isoGDGT pool.
The close relation between TEX86 and RI can also be used to detect aberrant distributions, including those produced by methanogenic, methanotrophic and terrestrial sources, as these sources typically contribute disproportionate amounts of specific lipids. RITEX, calculated from TEX using the polynomial fit of Zhang et al. (2016), is subtracted from the RI to arrive at the ΔRI. Cutoff values for sample deviation from the modern ocean calibration dataset are defined as 95 % confidence limits of the TEX86–RI relation, or above RI units.
2.3 H-shaped branched GDGTs; brGMGTs
BrGMGTs (Fig. 2) were first identified by Liu et al. (2012) in marine sediments, who identified a single acyclic tetramethylated brGMGT (m∕z 1020). This compound was later detected within the marine water column and appeared to be abundant within the oxygen minimum zone (Xie et al., 2014). Naafs et al. (2018a) identified a larger suite of brGMGTs (including m∕z 1048 and 1034) in a quasi-global compilation of modern peat samples. They argued that these compounds were preferentially produced at depth within the anoxic catotelm. Analogous to the continental paleothermometer based on bacterial brGDGTs produced in surface soils, termed MBT'5me (Weijers et al., 2007b; De Jonge et al., 2014), they showed that the degree of methylation of brGMGTs in peats relates to mean annual air temperature. They calculated the degree of methylation of brGDGTs without cyclopentane moieties, designed for comparison to the methylation of brGMGTs, defined by H-MBTacyclic:
Based on the strong relation between MBTacyclic and H-MBTacyclic in their peat samples, Naafs et al. (2018a) suggested that the brGMGTs have the same origin as the brGDGTs, presumably Acidobacteria (Sinninghe Damsté et al., 2011; Sinninghe Damsté et al., 2018a). In addition, they showed that the abundance of brGMGTs (relative to the total quantity of brGMGTs and brGDGTs) positively correlates with mean annual air temperature, suggesting that the covalent bond in the brGMGTs is used to maintain membrane stability at higher temperature (Naafs et al., 2018a).
Baxter et al. (2019) identified a total of seven different brGMGTs from a suite of African lake sediments (Fig. 2) and found their relative distribution to correlate with mean annual air temperature. Accordingly, they proposed a proxy for mean annual air temperature termed brGMGT-I (see Fig. 2 for the molecular structures referred to here):
We used the polar fractions previously analyzed by Sluijs et al. (2006, 2009) from the PETM through ETM2 interval at IODP Expedition 302 Hole 4A. These fractions originate from a total lipid extract produced using a Dionex accelerated solvent extractor and fraction separations by Al2O3 column chromatography using hexane:dichloromethane (DCM) (9:1, v∕v) and DCM:methanol (1:1; v∕v) to yield the apolar and polar fractions, respectively. Polar fractions were redissolved in hexane:isopropanol (99:1, v∕v) and passed through a 0.45 µm polytetrafluoroethylene filter. This fraction was then analyzed by high-performance liquid chromatography (HPLC) and atmospheric pressure chemical ionization–mass spectrometry using an Agilent 1260 Infinity series HPLC system coupled to an Agilent 6130 single-quadrupole mass spectrometer at Utrecht University following Hopmans et al. (2016) to measure the abundance of GDGTs. Based on long-term observation of the in-house standard, the analytical precision for TEX86 calculates to ±0.3 ∘C in the SST domain.
To gain further insights into the potential impact of terrestrial isoGDGT input on TEX86 values, we compiled isoGDGT and brGDGT distributions from modern peats (n=473; Naafs et al., 2017) and early Paleogene lignites (n=58; Naafs et al., 2018b). Note that the fractional abundance of crenarchaeol isomer was not reported in the early Paleogene dataset of Naafs et al. (2018b). We therefore revisited the original chromatograms from Naafs et al. (2018b) and integrated the crenarchaeol isomer (m∕z 1292).
The new GDGT distributions (Table S1 in the Supplement) are consistent with the TEX86 and BIT index data generated over a decade ago using the older analytical HPLC setup (Hopmans et al., 2000; Hopmans et al., 2016) (Fig. 3). TEX86 exhibits some scatter and the slope of the regression is slightly off the 1:1 line, indicating that the new data have somewhat higher TEX86 values. Less scatter is apparent in the BIT record, but the original BIT index values were slightly higher than recorded here, as indicated by the regression (Fig. 3). This result is consistent with previous analyses with the new analytical setup (Hopmans et al., 2016). This does not impact previous qualitative interpretations of this record (Sluijs et al., 2006; Sluijs et al., 2008b; Sluijs et al., 2009). In the Discussion section, we assess indicators of potential confounding factors (Sect. 2.2), including the influx of terrestrially derived isoGDGTs to the sediments (Figs. 4, 5 and S1) and several indices related to methane and depth of production (Fig. 6).
Although we did not detect significant quantities of isoprenoid GMGTs, high abundances of various brGMGTs are present in the ACEX samples, in total between 10 % and 45 % of the total brGDGT assemblage (Fig. 7). We consistently identify at least five brGMGTs across the three different mass-to-charge ratios (m∕z 1020, 1034 and 1048). Based on their (relative) retention times and overall distribution we were able to apply the nomenclature of Baxter et al. (2019) to five of these and assign individual peaks to previously identified compounds (Fig. S2). The abundance of brGMGTs relative to brGDGTs increases during the PETM. The proposed temperature indicators based on brGMGTs show mixed results, with some showing a clear response to the PETM (Fig. 7e) while others do not (Fig. 7d).
5.1 IsoGDGT provenance
5.1.1 Contributions of soil-derived isoGDGTs
As noted by Sluijs et al. (2006), late Paleocene samples yield anomalously high abundances of GDGT-3, likely derived from a terrestrial source. We therefore consider the late Paleocene temperature estimates unreliable. To assess the temperature change during the PETM, Sluijs et al. (2006) developed a TEX86 calibration without this moiety, termed TEX. However, TEX has not been widely used outside the Paleogene Arctic because the anomalous abundances of GDGT-3 have not been recorded elsewhere. High contributions of GDGT-3 from terrestrial input would also be associated with an increase in the abundance of other isoGDGTs. Indeed, recent TEX86-based global SST compilations and comparisons to climate simulations for the PETM excluded the Paleocene ACEX data because the TEX calibration complicates the comparison to other regions where it has not been applied (Frieling et al., 2017; Hollis et al., 2019).
Input of soil organic matter is consistent with Willard et al. (2019), who established that the brGDGT assemblage is dominantly soil-derived as opposed to being produced in the coastal marine environment. This observation is based upon the weighted average number of rings in the tetramethylated brGDGTs (#ringstetra), which generally does not exceed 0.4 to 0.7 in the global soil calibration dataset (Sinninghe Damsté, 2016). In the ACEX record, #ringstetra is < 0.21 (Willard et al., 2019), consistent with a dominant soil source. This indicates that (1) brGDGT abundances, (2) brGDGT distributions and (3) the BIT index are reliable indicators of the relative supply of terrestrially derived isoGDGTs into the marine basin. The Paleocene section of the dataset also stands out regarding its relation between the BIT index and TEX86 (Fig. 4), which confirms its anomalous nature.
During the PETM, TEX86 values are higher (due to warming) and BIT values lower. This was attributed to sea level rise during the hyperthermals, resulting in a more distal position relative to the terrestrial GDGT source (Sluijs et al., 2006; Sluijs et al., 2008a). The interval between 371.0 and 369.0 mcd (i.e., above the PETM and below ETM2) also stands out. This interval was previously recognized by Sluijs et al. (2009) to reflect an open marine environment, with a dominance of marine palynomorphs and algal biomarkers. They also found that high BIT values correspond to low TEX86 values within that interval and therefore implemented a subjective threshold value of 0.3, above which TEX86-derived SSTs were considered unreliable. Although the relation between BIT and TEX86 exhibits considerable scatter, the new analyses support the notion that a higher influx of terrestrial isoGDGTs lowers TEX86 values. The linear regression (Fig. 4; excluding the one outlier with high TEX86 and BIT values in the top right of the plot because it has highly anomalous distributions; ΔRI = 0.61) yields an R2 of 0.26 that explains a portion of the variation (Fig. 4). The nature of this influence is determined by the relative abundance of terrestrial isoGDGTs and their TEX86 value. The TEX86 value at the terrestrial endmember of BIT = 1, assuming various types of regressions, centers around 0.5. The remainder of the data does not show a clear relation between BIT and TEX86, although some of the lowest TEX86 values correspond to high BIT values, suggesting that the terrestrial endmember contributed isoGDGT assemblages with relatively low TEX86 values in other intervals as well.
The relatively low degree of cyclization in the early Eocene contrasts starkly with the high degree of cyclization during the late Paleocene (Fig. 6e). This implies that the distribution of terrestrial isoGDGTs varies strongly between the latest Paleocene and early Eocene within our studied section.
The impact of soil-derived isoGDGTs also emerges from the ring index approach of Zhang et al. (2016; see Sect. 2.2.6 and Fig. 6). The difference between the ring index and TEX86 at the onset of the PETM is mainly controlled by crenarchaeol, which is comparatively low in abundance in the Paleocene but highly abundant in the PETM. This increase is likely associated with sea level rise during the PETM because crenarchaeol is predominantly produced in the marine realm. It is also consistent with a drop in BIT index values and the relative abundance of terrestrial palynomorphs (Sluijs et al., 2008a). The approach of Zhang et al. (2016) also confirms that many isoGDGT distributions exhibit an anomalous relation between TEX86 and the ring index relative to the modern core top dataset, with ΔRI values > 0.3 (Fig. 6). Importantly, all samples with ΔRI values > 0.3 have BIT values above 0.35, indicating that contributions of soil-derived iso-GDGTs dominate non-temperature effects in the distributions. We therefore discard TEX86-derived SSTs for samples with BIT values > 0.35.
We also develop a crude model to further constrain the potential contribution of terrestrially derived isoGDGTs. First, we determine the abundance of isoGDGTs relative to brGDGTs in modern peat samples (Naafs et al., 2017) and early Paleogene lignites (fossil peat) (Naafs et al., 2018b; the isoGDGT data are published here). Although there is no reason to assume that peat was a major component of the hinterland (Willard et al., 2019), the aforementioned datasets can provide an estimate of the potential contribution from terrestrial isoGDGTs to our study site. The raw signal intensity of brGDGTs in the ACEX samples is used to estimate the potential contribution of terrestrially derived isoGDGTs to the samples. To this end, we use the fractional abundance of the various isoGDGTs in the modern peat and Paleogene lignite datasets (Fig. 5). Then, we estimate the abundance of these terrestrially derived isoGDGTs in our ACEX samples by scaling this fraction to the measured abundances of brGDGTs and isoGDGTs in our ACEX samples, following
where x represents the specific analyzed GDGT (see the Supplement for an example of these calculations).
This leads to estimates of the potential relative contributions of the individual isoGDGTs derived from land to the ACEX samples based on the entire modern peat dataset (Naafs et al., 2017), modern peats from regions with mean annual air temperature (MAT) exceeding 15 ∘C (Naafs et al., 2017) and Paleogene lignites (Naafs et al., 2018b; this paper, Figs. 5 and S1). This approach implies that crenarchaeol and the crenarchaeol isomer are almost exclusively from the marine realm. However, GDGT-1, GDGT-2 and GDGT-3 in our study site may be derived from the terrestrial realm (Fig. 5), especially in specific stratigraphic intervals (Fig. S1). In the most extreme cases, the modeled contributions of terrestrial isoGDGTs are higher than the measured isoGDGT abundances (i.e., terrestrial fraction > 1). This is principally seen in isoGDGT-2 and GDGT-3, especially when we employ the Paleogene lignite database. This particular assumption clearly overestimates the abundance of terrestrially sourced isoGDGTs in our setting. However, the temporal trends obtained using modern peats, subtropical modern peats and Paleogene lignites are essentially identical and give some indication of which isoGDGTs are most likely to be impacted by terrestrial input and across which intervals. Interestingly, this approach also suggests that particularly GDGT-3 is shown to be strongly affected (Fig. 5), which qualitatively matches the distributions in the ACEX samples. This is principally because GDGT-3 is the least abundant marine isoGDGT included in our analyses, whereas it is often as abundant as GDGT-1 and GDGT-2 in terrestrial settings (Fig. 5).
5.1.2 Contributions of methanotrophic or methanogenic archaea?
The depositional environment at the study site included ample (export) production, sediment organic matter content and low-oxygen conditions at the sediment–water interface (Sluijs et al., 2006; Stein et al., 2006; Stein, 2007; Sluijs et al., 2008b; Sluijs et al., 2009; März et al., 2010). This may have been suitable for abundant methanogenic and methanotrophic archaea, potentially contributing to the sedimentary isoGDGT assemblage. However, our GDGT-2 ∕ crenarchaeol values (< 0.23; Fig. 6) are far below values that suggest significant isoGDGT contributions of methanotrophic Euryarchaeota as described by Weijers et al. (2011). MI values (maximum observed 0.31) are also generally below proposed cutoff values (0.3–0.5, Zhang et al., 2011) that suggest such contributions. Finally, GDGT-0 ∕ crenarchaeol ratios (< 1.4) remain below the cutoff value of 2 throughout the section (Fig. 6), also making a significant isoGDGT contribution from methanogens highly unlikely (Blaga et al., 2009). Collectively, relative contributions of isoGDGTs from methanogenic and methanotrophic archaea seem low despite the low-oxygen environment, suggesting a relatively high flux of pelagic isoGDGTs.
5.1.3 Contributions of deep-dwelling archaea?
Taylor et al. (2013) showed that GDGT-2 ∕ GDGT-3 ratios correspond to depth of production, with high values (> 5) in deep waters (> 1000 m). We record low values (1–4) between ∼ 390 and ∼ 371.2 mcd (Fig. 6), which supports dominant production in the surface ocean based on the modern calibration dataset (Taylor et al., 2013). However, the overlying interval (∼ 371 to ∼ 368.3 mcd) has much higher (average 7.4) and variable GDGT-2 ∕ GDGT-3 values, with peak values of 10–14. Such values suggest significant contributions of isoGDGTs produced at water depths of several kilometers according to the analyses by Taylor et al. (2013).
However, all paleoenvironmental information generated based on the sediments and tectonic reconstructions of Lomonosov Ridge – a strip of continental crust that disconnected from the Siberian margin in the Paleocene – has indicated a neritic setting of the drill site at least up to the middle Eocene (e.g., O'Regan et al., 2008; Sangiorgi et al., 2008; Sluijs et al., 2008a; Sluijs et al., 2009). At ∼ 371.2 mcd a drop in the BIT index and a change in the palynological assemblages correspond to an interval of greenish sediment suggestive of pronounced amounts of glauconite. These changes are consistent with local relative sea level rise, causing a somewhat more distal position relative to the shoreline. However, the sediment remains dominantly siliciclastic and organic terrestrial components, particularly pollen and spores, still remain abundant, indicating a shallow setting (Sluijs et al., 2008a, 2008b). Increased contributions of isoGDGTs produced at depth would be expected to have caused a systematic cold bias, but based on linear regression analysis the large variability in GDGT-2 ∕ GDGT-3 ratios is unrelated to the recorded variability in TEX86 values. The high GDGT-2 ∕ GDGT-3 ratio values therefore cannot be explained by contributions of deep-dwelling archaea.
In a study of the last 160 kyr in the South China Sea, Dong et al. (2019) found that very high GDGT-2 ∕ GDGT-3 ratios (∼ 9 but up to 13) correspond to high values in nitrogen isotope ratios, interpreted to reflect low contributions in diazotroph N2 fixation and enhanced upwelling. In our record, the high GDGT-2 ∕ GDGT-3 ratios are associated with normal marine conditions and the dinocyst assemblages are not indicative of upwelling conditions (Sluijs et al., 2009). Unfortunately, the available nitrogen isotope record (Knies et al., 2008) does not cover this interval in sufficient resolution to assess a relation with diazotroph activity. The increase in the GDGT-2 ∕ GDGT-3 ratio correlates with a strong drop in BIT index values and an increase in normal marine dinocyst species (Sluijs et al., 2009), but a shift to more open marine environment does not explain the high ratio values. As such, the cause of the high GDGT-2 ∕ GDGT-3 ratios in this interval remains unclear, but we consider it highly unlikely to relate to contributions of deep-dwelling Thaumarchaeota.
5.1.4 Oxygen concentrations and ammonium oxidation rates
A variety of nonthermal factors can impact TEX86 values, including ammonium and oxygen concentrations and growth phase (Elling et al., 2014; Qin et al., 2014; Hurley et al., 2016). Across the studied interval of the ACEX core, several intervals of seafloor and water column anoxia have been identified based on organic and inorganic proxies, notably during the PETM and ETM2 (Sluijs et al., 2006; Stein et al., 2006; Sluijs et al., 2008b; Sluijs et al., 2009; März et al., 2010).
Particularly suspect is an interval of low TEX86 values that marks the middle of the ETM2 interval, directly following a ∼ 4 ∘C warming at its onset (Sluijs et al., 2009). This interval is also marked by the presence of sulfur-bound isorenieratane (Sluijs et al., 2009), a derivative of isorenieratene. This biomarker is produced by the brown strain of green sulfur bacteria that require light for photosynthesis and free sulfide, indicating euxinic conditions in the (lower) photic zone (Sinninghe Damsté et al., 1993). We also record a concomitant shift in several methane-related indicators, GDGT-2 ∕ GDGT-3 ratio values and the ΔRI. A mid-ETM2 cooling signal has not been recorded at other study sites, and this interval marks the occurrence of pollen of thermophilic plants such as palms and baobab (Sluijs et al., 2009; Willard et al., 2019). Therefore, the low TEX86 values were suggested to reflect thaumarchaeotal depth migration to the deeper chemocline due to euxinic conditions (Sluijs et al., 2009), similar to the modern Black Sea (Coolen et al., 2007; Wakeham et al., 2007) and the Mediterranean Sea during sapropel formation (Menzel et al., 2006).
More recent work has indicated that the isolated marine thaumarchaeotal species Nitrosopumilus maritimus produces lower TEX86 values with higher ammonia oxidation rates (Hurley et al., 2016) and O2 concentrations (Qin et al., 2015). Although this observation is difficult to extrapolate to the total response of the thaumarchaeotal community in the marine environment on geological timescales, lower O2 availability should lower oxidation rates, leading to higher TEX86 values (Qin et al., 2015; Hurley et al., 2016). However, we record a drop in TEX86 values with the development of anoxia during ETM2. The nature of the anomalously low cyclization in the ETM2 isoGDGT assemblage, which passes all quality tests regarding GDGT distribution (Fig. 6), therefore remains elusive.
5.2 Origin and environmental forcing of brGMGTs
The relative abundances of brGMGTs in our samples are surprisingly high. On average, they comprise 25 % of the total branched GDGT and GMGT assemblage. The limited literature on modern occurrences implies that both terrestrial and marine sources may have contributed to the brGMGT assemblage. Data from marine sediments (Liu et al., 2012) and the water column (Xie et al., 2014) clearly show production within the marine realm. Their occurrence in modern peats (Naafs et al., 2018a), lake sediments (Baxter et al., 2019) and Paleogene lignites (Inglis et al., 2019) might also imply transport from land to marine sediments. A soil-derived source is currently unsupported, as they were most often below the detection limit in recent studies of geothermally heated soils (De Jonge et al., 2019) and a soil transect from the Peruvian Andes (Kirkels et al., 2020). The relative brGMGT abundances we record are close to the maximum found in modern peats (Naafs et al., 2018a). However, significant input of peat-derived organic matter into our study site is inconsistent with the low input of peat-derived Sphagnum spores (Willard et al., 2019). Alternatively, the high abundance of brGMGTs could also be related to subsurface production in marine sediments. An analogous process was invoked by Naafs et al. (2018a) to explain the very high abundance of brGMGTs in an early Paleogene lignite. Collectively, however, we surmise that production in the marine realm may be an important contributor to the brGMGT pool in our setting.
Several factors may contribute to the rise in the abundance of brGMGTs relative to brGDGTs across the PETM. Higher relative abundances of brGMGTs in modern peats generally occur at higher mean annual air temperatures (Naafs et al., 2018a), so this signal could relate to warming during the PETM if their origin at the study site is terrestrial. However, since we consider it likely that a large part of the brGMGT assemblage is of marine origin, the rise in brGMGT abundance likely relates to the previously recorded (Sluijs et al., 2006; Sluijs et al., 2008b) sea level rise during the PETM at the study site. This is consistent with the increase in marine brGMGT production relative to terrestrial brGDGT supply to the study site (Fig. 7b). This is consistent with the inverse correlation between brGMGT abundance and the BIT index (Fig. 7b). Lastly, if the production of marine brGMGTs was focused in oxygen minimum zones (Xie et al., 2014), the development of low-oxygen conditions in the water column based on several indicators, such as the presence of isorenieratane (Sluijs et al., 2006), might have increased the production of brGMGTs in the water column. It is also possible that all of these factors contributed to the changes in the abundance of brGMGTs relative to brGDGTs across the PETM.
The brGMGT-I proxy does not produce temperature trends similar to those seen in TEX86 or MBT'5me (Fig. 7d). If the majority of the brGMGTs are of marine origin, this indicates that brGMGTs produced in the marine realm do not respond to temperature as was hypothesized based on the African lake dataset by Baxter et al. (2019).
Also, the application of the H-MBTacyclic index (equation 7) appeared problematic because, similar to Baxter et al. (2019), we identified several more isomers than Naafs et al. (2018a, who developed this index) detected in their peat samples. It therefore remains unclear which of our peaks should be used to calculate the H-MBTacyclic index values. We therefore show the two plausible options. For the first, we use all peaks with m∕z 1020, 1034 and 1048 (H-MBT-all in Fig. 7e) within the expected retention time window. However, based on our chromatography, we consider it more likely that the dominant peaks identified by Naafs et al. (2018a) at m∕z 1020 and 1034 represent H1020c and H1034b, respectively, and therefore use only those in addition to the single identifiable peak at m∕z 1048 as a second option (H-MBT; H1020c, H1034b) in Fig. 7e. Both options show a clear rise across the PETM, although the HMBT (H1020c, H1034a) shows a larger signal and somewhat better correspondence in absolute values to MBTacyclic though with more scatter. A close correspondence between MBTacyclic and HMBT has also been found in a lignite that has been assigned to the PETM (Inglis et al., 2019).
If the dominant source of the brGMGTs was marine throughout the record, the increase in methylation possibly relates to warming. This would not be unprecedented as marine-produced brGDGTs show an increase in methylation as a function of temperature (Dearing Crampton-Flood et al., 2018). Sollic et al. (2017) also suggest that archaeal-derived isoprenoid GMGTs produced in marine sediments incorporate additional methyl groups at higher sediment temperatures. Water column oxygen concentrations and pH also changed at our site during the PETM, which potentially affected distributions. Extensive evaluation of brGMGT distributions in modern samples is therefore required to assess the proxy potential.
5.3 Uncertainty in TEX86-based SST estimates
5.3.1 Uncertainty based on calibration dataset
To calculate SSTs, we use (1) the BAYSPAR method (Tierney and Tingley, 2014), which assumes a linear relationship between TEX86 and SST, and (2) (Kim et al., 2010), which assumes a nonlinear relationship between TEX86 and SST. Differences between these calibrations are smaller than the calibration errors (Fig. 6) because the TEX86 values in the ACEX dataset all fall within the range of the modern core-top calibration. Taken together, both indices imply that mean annual SSTs varied between 18 and 28 ∘C in the early Eocene, providing strong evidence for remarkable early Eocene warmth in the Arctic region.
The TEX calibration has a calibration error of 2.5 ∘C (residual mean standard error; RMSE) (Kim et al., 2010). The BAYSPAR method yields possible values that range ± 6 ∘C from the most probable value (Fig. 6), but these uncertainty estimates are more comparable than is immediately apparent, as this analysis takes a 90 % confidence interval compared to the 68 % probability of RMSE. All of the calibrations and methods to obtain values and uncertainties are based on a modern core-top dataset and thus implicitly include potential confounding factors such as seasonality and depth of production and export. However, there is no (quantitative) constraint on any of these parameters in the calibration dataset. This is particularly important for the studied region because it represents a polar endmember of the marine environment with highly seasonal production and export as well as potentially high seasonality in temperature. In the modern ocean, relations between SST and TEX86 in the Arctic and ice-proximal Southern Ocean settings differ from the global ocean. This is attributed to a change in viscoelastic adaptation to temperature at the low end and/or a change in the thaumarchaeotal community (Kim et al., 2010; Ho et al., 2014; Tierney and Tingley, 2014). This may mask potential confounding factors that may be specifically relevant to polar environments. This is important here, where the polar regions were ice-free and the functioning of physical, chemical and biological ocean systems was fundamentally different from the present day. This uncertainty is not accounted for using traditional regression analyses or Bayesian techniques, and quantification of uncertainty in non-analog climates remains extremely difficult.
5.3.2 Constraints from independent proxy data
Independent proxy data may provide additional constraints. The appearance of the dinoflagellate cyst genus Apectodinium during the PETM and ETM2 in the Arctic basin (Sluijs et al., 2006; Sluijs et al., 2009; Harding et al., 2011) provides qualitative support for pronounced warming and apparent subtropical conditions. Recent efforts to quantify the paleoecological affinities of this now extinct genus have suggested a required minimum temperature of ∼ 20 ∘C (Frieling et al., 2014; Frieling and Sluijs, 2018). Although this value is partly based on TEX86 data from the ACEX cores, it is supported by data from an epicontinental site in Siberia (Frieling et al., 2014).
A second line of independent proxy evidence includes vegetation reconstructions. As indicated above, the TEX86 results are qualitatively consistent with the ample evidence for thermophilic plants and animals in the Arctic (e.g., Heer, 1869; Schweitzer, 1980; Greenwood and Wing, 1995; Uhl et al., 2007; Suan et al., 2017). Particularly valuable are minimum winter temperature tolerances for specific plant species. Palynological analyses have indicated the presence of palm and baobab pollen within the PETM and ETM2 intervals in the ACEX cores (Sluijs et al., 2009; Willard et al., 2019). Modern palms are unable to tolerate sustained intervals of frost, and sexual reproduction is limited to regions where the coldest-month mean temperature (CMMT) is significantly above freezing (Van der Burgh, 1984; Greenwood and Wing, 1995). This threshold was recently quantified to be ≥ 5.2 ∘C (Reichgelt et al., 2018). The presence of baobab within the PETM interval and ETM2 also indicates mean winter air temperatures of at least 6 ∘C (Willard et al., 2019). Importantly, these plants were not encountered in the intervals outside the PETM and ETM2, suggesting that background coldest-month mean air temperatures were potentially too low (< 6 ∘C) to support megathermal vegetation.
Pollen of palms and Avicennia mangroves was recently identified in time-equivalent sections in Arctic Siberia (Suan et al., 2017). Although the details of the stratigraphic framework for these records may be somewhat problematic, these findings indicate elevated CMMT estimates on land (> 5.5 ∘C) and in the surface ocean (> 13 ∘C) during the late Paleocene and early Eocene (Suan et al., 2017).
Apparently conflicting evidence comes from the occurrence of glendonites and erratics in specific stratigraphic levels in Paleocene and Eocene strata in Spitsbergen, interpreted to reflect “cold snaps” in climate (Spielhagen and Tripati, 2009). Some of these stratigraphic levels are very close to (or even potentially within) the PETM, considering the local stratigraphic level of the PETM (Cui et al., 2011; Harding et al., 2011). However, glendonites and erratics have not been found at the exact same stratigraphic levels as thermophilic biota (Spielhagen and Tripati, 2009). The formation and stability of ikaite (the precursor mineral of the diagenetic glendonites) in Spitsbergen were dependent on relatively low-temperature, arguably persistent near-freezing seawater temperatures in the sediment (Spielhagen and Tripati, 2009). However, glendonite occurrences in other settings (e.g., Mesozoic sediments in midlatitude regions; Teichert and Luppold, 2013) have also recently been linked to methane seeps (Morales et al., 2017). Therefore, the specific temperature constraints implied by glendonites under such conditions are the subject of debate. Future work should apply temperature reconstructions based on the geochemical composition of the glendonites and biomarkers or biota on corresponding strata to assess whether glendonite occurrence is related to colder climates.
The estimate on seasonal minima provides an important constraint on Arctic climatology during the PETM and ETM2. Most likely, the palms and baobabs grew close to the shore, where the relative heat of the ocean kept atmospheric temperatures relatively high during the winter. If minimum winter SSTs were in the range of the SST reconstructions based on the nearby Avicennia mangrove pollen (Suan et al., 2017), which for open ocean settings would perhaps amount to ∼ 10 ∘C, then summer SST must have soared to at least 30 ∘C in summer if TEX86-based SST reconstructions of ∼ 20 ∘C truly reflect the annual mean. It would imply an SST seasonality of ∼ 20 ∘C, much higher than any modern open marine setting. In the present-day Arctic Ocean, heat is seasonally stored and released in sea ice melting and freezing, and sea ice cover insulates the ocean and reflects a high amount of sunlight, resulting in a seasonal cycle of not more than 1.5 ∘C, even in ice-free regions (Chepurin and Carton, 2012). However, coupled model simulations have indicated that the future loss of sea ice will greatly enhance the seasonal SST range to up to 10 ∘C in 2300 given unabated CO2 emissions (Carton et al., 2015). With year-round snow- and ice-free conditions as well as even stronger summer stratification during the Eocene due to higher greenhouse gas concentrations and freshwater supply through an enhanced hydrological cycle (Pierrehumbert, 2002; Carmichael et al., 2017), a nearshore 20 ∘C seasonal cycle in Arctic Ocean SST may not be unrealistic, although it remains inconsistent with current-generation fully coupled, relatively low-resolution model simulations (e.g., Frieling et al., 2017).
Constraints from the total pollen assemblages in the ACEX cores based on a nearest living relative approach suggest Arctic mean annual temperatures on land of 13–18 ∘C and summer temperatures significantly exceeding 20 ∘C during the PETM and ETM2 (Willard et al., 2019). Although these estimates come with much larger uncertainty than winter temperatures and may suffer from the non-analogous setting, they are generally lower than our TEX86 values. The brGDGT-based paleothermometer MBT'5me (De Jonge et al., 2014) also suggests lower mean annual air temperatures than reported from TEX86 (Willard et al., 2019, Fig. 7). These data, derived from the same HPLC–MS analyses as the isoGDGT data presented here, indicate mean annual air temperatures averaging ∼ 18 ∘C during the PETM, with a residual mean calibration error of 4.8 ∘C. This value is ∼ 7 ∘C lower than earlier estimates based on a slightly different method and analytical procedure as well as a smaller modern calibration dataset (Weijers et al., 2007a). However, recent inclusion of data from Indian soils in the MBT'5me calibration dataset improved the proxy at the higher-temperature end (Dearing Crampton-Flood et al. 2020). The new calibration, BayMBT, results in air temperatures ∼ 3 ∘C higher than that of De Jonge et al. (2014), which was used by Willard et al. (2019; Fig. 7). The high calibration uncertainty of MBT (see the Supplement) implies that the biomarker-derived air temperature and SST reconstructions are within error.
5.4 State of constraints on Paleocene–Eocene Arctic temperatures
To unlock the unique premise of Eocene climates for testing the skill of current-generation fully coupled climate models under high greenhouse gas forcing, proxy data and models are ideally approached separately. Among the most important implications of the Arctic temperature estimates are reconstructions of the meridional temperature gradients. Importantly, not a single simulation using an IPCC-class model of early Paleogene climate has produced Arctic annual mean sea surface temperatures close to the ACEX TEX86-based reconstructions without unrealistically high tropical SSTs (Lunt et al., 2012). Recent simulations using the Community Earth System Model (CESM) versions 1 (Frieling et al., 2017; Cramwinckel et al., 2018) and 1.2 (Zhu et al., 2019) with Eocene boundary conditions produced climates that correspond to SST reconstructions in many ocean regions based on several proxies, but they still produced cooler mean annual SSTs for the Arctic Ocean than suggested by TEX86 (Frieling et al., 2017; Cramwinckel et al., 2018; Zhu et al., 2019). TEX86 also indicates SSTs higher than in these model simulations at several sites along the Antarctic margin (Bijl et al., 2009; Bijl et al., 2013). The question thus remains of whether the conversion of TEX86 values to mean annual SST using any modern core-top calibration for high-latitude Paleogene locations is valid and whether the climate models still significantly underestimate polar temperatures. Certainly, if interpreted as mean annual SST, TEX86-based estimates are high compared to the few available additional estimates, notably based on vegetation, but the latter also suffer from similar uncertainties (e.g., Hollis et al., 2019).
A few biases might lead to underestimates of meridional temperature gradients as indicated from TEX86. First, the flat Eocene temperature gradient implied by TEX86 was suggested to result from erroneously calibrating the proxy to SST rather than to the temperature of the subsurface (Ho and Laepple, 2016). The rationale is that the meridional temperature gradient is smaller in deeper waters than it is in the surface. However, the idea was contested for multiple reasons, including the fact that sediments at most Eocene study sites, such as the ACEX site, were deposited at a depth of less than 200m, making the application of a deep subsurface (> 1000 m) calibration inappropriate (Tierney et al., 2017). Moreover, recent analyses have indicated that the TEX86 signal dominantly reflects the temperature of the top 200 m of the water column (Zhang and Liu, 2018).
Secondly, as suggested previously (Sluijs et al., 2006), if TEX86 were biased towards any season in the non-analog Arctic Ocean, it would be the summer, the dominant season of organic matter export towards the seafloor through fecal pelleting or marine snow aggregates. Vegetation suggests very high winter continental coldest-month mean air temperatures of at least 6–8 ∘C (Sluijs et al., 2009; Suan et al., 2017; Willard et al., 2019), coastal coldest-month mean SSTs of > 13 ∘C (Suan et al., 2017), and terrestrial mean annual and warmest-month mean temperature on land of 13–21 ∘C and > 20 ∘C, respectively (Suan et al., 2017; Willard et al., 2019) (see Sect. 5.3.2). These estimates are closer to the most recent model simulations and lower than the existing TEX86 (e.g., Frieling et al., 2017; Zhu et al., 2019). If TEX86-implied SST of ∼ 25 ∘C is skewed towards a summer estimate, this would decrease the model–data bias regarding the meridional temperature gradient estimates. Given the current uncertainties in the use of TEX86 for the non-analog Arctic Ocean, we cannot independently constrain this.
We analyzed isoGDGT and brGMGT (H-shaped brGDGT) distributions in sediments recovered from the Paleocene–Eocene Thermal Maximum (PETM; ∼ 56 Ma) to Eocene Thermal Maximum 2 (ETM2; ∼ 54 Ma) interval on Lomonosov Ridge, Arctic Ocean, using state-of-the-art analytical procedures, compared them to the original dataset (Sluijs et al., 2006; Sluijs et al., 2009) and interpreted the results following the currently available TEX86 proxy constraints.
Although contributions of isoGDGTs from land complicate TEX86 paleothermometry in some stratigraphic intervals, temperature was the dominant variable controlling TEX86 values. Background early Eocene SSTs exceed ∼ 20 ∘C, and peak warmth occurred during the PETM and ETM2. However, uncertainty estimates of these SSTs based on the non-analog modern ocean remain complex. Temperature constraints from terrestrial vegetation support remarkable warmth in the study section and elsewhere in the Arctic basin, notably coldest-month mean temperatures around 10 ∘C at least within the PETM and ETM2. If TEX86-derived SSTs of ∼ 20 ∘C truly represent mean annual SSTs, the seasonal range of Arctic SST might have been of the order of 20 ∘C. If SST estimates are entirely skewed towards the summer season, seasonal ranges of the order of 10 ∘C may be considered comparable to those simulated in future ice-free Arctic Ocean scenarios.
We find abundant brGMGTs, which appear to be predominantly produced in the marine realm at the study site. Their abundance increases during the PETM, likely due to sea level rise and perhaps due to warming and a drop in seawater oxygen concentrations. Although speculative, an increase in brGMGT methylation during the PETM may be a function of temperature, but a relation between brGMGT distribution and environmental parameters including temperature has yet to be confirmed.
All data are provided in Table S1 and stored in the Pangaea database (https://doi.org/10.1594/PANGAEA.924854, Sluijs, 2020).
Requests for materials can be addressed to Appy Sluijs (email@example.com).
The supplement related to this article is available online at: https://doi.org/10.5194/cp-16-2381-2020-supplement.
AS initiated the study, KGJN generated the data and JF modeled terrestrial contributions of isoGDGTs based on published information and the new crenarchaeol data from the modern peat dataset, which was contributed by GNI. All authors contributed to the interpretation of the data, and AS wrote the paper with input from all authors.
The authors declare that they have no conflict of interest.
We thank the ACEX scientific party for collaborations over the past 16 years, the International Ocean Discovery Program (IODP) for access to ACEX samples and data, and the Dutch Research Council (NWO) for their continued support to IODP. We thank Linda van Roij for analytical support, Emily Dearing Crampton-Flood for providing BayMBT results and David Naafs for providing the original chromatograms published in Naafs et al. (2018b).
This research has been supported by the European Research Council (grant no. SPANC 771497 to AS), the Ministry of Education, Culture and Science of the Netherlands (grant from the Netherlands Earth System Science Centre), and a GCRF Royal Society Dorothy Hodgkin Fellowship (to GNI).
This paper was edited by Zhengtang Guo and reviewed by Tom Dunkley Jones and one anonymous referee.
Backman, J., Moran, K., McInroy, D. B., Mayer, L. A., and Expedition-302-Scientists: Proceedings of the Integrated Ocean Drilling Program, Integrated Ocean Drilling Program Management International, Inc., Edinburgh, 302, https://doi.org/10.2204/iodp.proc.302.2006, 2006.
Baxter, A. J., Hopmans, E. C., Russell, J. M., and Sinninghe Damsté, J. S.: Bacterial GMGTs in East African lake sediments: Their potential as palaeotemperature indicators, Geochim. Cosmochim. Ac., 259, 155–169, https://doi.org/10.1016/j.gca.2019.05.039, 2019.
Berry, E. W.: A Possible Explanation of Upper Eocene Climates, P. Am. Philos. Soc., 61, 1–14, 1922.
Besseling, M. A., Hopmans, E. C., Bale, N. J., Schouten, S., Damsté, J. S. S., and Villanueva, L.: The absence of intact polar lipid-derived GDGTs in marine waters dominated by Marine Group II: Implications for lipid biosynthesis in Archaea, Sci. Rep.-UK, 10, 294, https://doi.org/10.1038/s41598-019-57035-0, 2020.
Bijl, P. K., Schouten, S., Sluijs, A., Reichart, G.-J., Zachos, J. C., and Brinkhuis, H.: Early Palaeogene temperature evolution of the southwest Pacific Ocean, Nature, 461, 776–779, 2009.
Bijl, P. K., Bendle, J. A. P., Bohaty, S. M., Pross, J., Schouten, S., Tauxe, L., Stickley, C. E., McKay, R. M., Rohl, U., Olney, M., Sluijs, A., Escutia, C., Brinkhuis, H., and the Expedition 318 Scientists: Eocene cooling linked to early flow across the Tasmanian Gateway, P. Am. Philos. Soc., 110, 9645–9650, https://doi.org/10.1073/pnas.1220872110, 2013.
Blaga, C. I., Reichart, G.-J., Heiri, O., and Sinninghe Damsté, J. S.: Tetraether membrane lipid distributions in water-column particulate matter and sediments: a study of 47 European lakes along a north–south transect, J. Paleolimnol., 41, 523–540, https://doi.org/10.1007/s10933-008-9242-2, 2009.
Carmichael, M. J., Inglis, G. N., Badger, M. P. S., Naafs, B. D. A., Behrooz, L., Remmelzwaal, S., Monteiro, F. M., Rohrssen, M., Farnsworth, A., Buss, H. L., Dickson, A. J., Valdes, P. J., Lunt, D. J., and Pancost, R. D.: Hydrological and associated biogeochemical consequences of rapid global warming during the Paleocene-Eocene Thermal Maximum, Global Planet. Change, 157, 114–138, https://doi.org/10.1016/j.gloplacha.2017.07.014, 2017.
Carton, J. A., Ding, Y., and Arrigo, K. R.: The seasonal cycle of the Arctic Ocean under climate change, Geophys. Res. Lett., 42, 7681–7686, https://doi.org/10.1002/2015GL064514, 2015.
Chepurin, G. A. and Carton, J. A.: Subarctic and Arctic sea surface temperature and its relation to ocean heat content 1982–2010, J. Geophys. Res.-Oceans, 117, C06019, https://doi.org/10.1029/2011JC007770, 2012.
Coolen, M. J. L., Abbas, B., v. Bleijswijk, J., Hopmans, E. C., Kuypers, M. M. M., Wakeham, S. G., and Damsté, J. S. S.: Putative ammonia-oxidizing Crenarchaeota in suboxic waters of the Black Sea: a basin-wide ecological study using 16S ribosomal and functional genes and membrane lipids, Environ. Microbiol., 9, 1001–1016, 2007.
Cramwinckel, M. J., Huber, M., Kocken, I. J., Agnini, C., Bijl, P. K., Bohaty, S. M., Frieling, J., Goldner, A., Hilgen, F. J., Kip, E. L., Peterse, F., van der Ploeg, R., Röhl, U., Schouten, S., and Sluijs, A.: Synchronous tropical and polar temperature evolution in the Eocene, Nature, 559, 382–386, https://doi.org/10.1038/s41586-018-0272-2, 2018.
Cui, Y., Kump, L. R., Ridgwell, A. J., Charles, A. J., Junium, C. K., Diefendorf, A. F., Freeman, K. H., Urban, N. M., and Harding, I. C.: Slow release of fossil carbon during the Palaeocene-Eocene Thermal Maximum, Nat. Geosci., 4, 481–485, 2011.
Dawson, M. R., West, R. M., Langston Jr., W., and Hutchison, J. H.: Paleogene terrestrial vertebrates: Northernmost occurrence, Ellesmere Island, Canada, Science, 192, 781–782, 1976.
De Jonge, C., Hopmans, E. C., Zell, C. I., Kim, J.-H., Schouten, S., and Sinninghe Damsté, J. S.: Occurrence and abundance of 6-methyl branched glycerol dialkyl glycerol tetraethers in soils: Implications for palaeoclimate reconstruction, Geochim. Cosmochim. Ac., 141, 97–112, https://doi.org/10.1016/j.gca.2014.06.013, 2014.
De Jonge, C., Radujković, D., Sigurdsson, B. D., Weedon, J. T., Janssens, I., and Peterse, F.: Lipid biomarker temperature proxy responds to abrupt shift in the bacterial community composition in geothermally heated soils, Org. Geochem., 137, 103897, https://doi.org/10.1016/j.orggeochem.2019.07.006, 2019.
Dearing Crampton-Flood, E., Peterse, F., Munsterman, D., and Sinninghe Damsté, J. S.: Using tetraether lipids archived in North Sea Basin sediments to extract North Western European Pliocene continental air temperatures, Earth Planet. Sci. Lett., 490, 193–205, https://doi.org/10.1016/j.epsl.2018.03.030, 2018.
Dearing Crampton-Flood, E., Tierney, J. E., Peterse, F., Kirkels, F. M. S. A., and Sinninghe Damsté, J. S.: BayMBT: A Bayesian calibration model for branched glycerol dialkyl glycerol tetraethers in soils and peats, Geochim. Cosmochim. Ac., 268, 142–159, https://doi.org/10.1016/j.gca.2019.09.043, 2020.
De Rosa, M., Esposito, E., Gambacorta, A., Nicolaus, B., and Bu'Lock, J. D.: Effects of temperature on ether lipid composition of Caldariella acidophila, Phytochemistry, 19, 827–831, https://doi.org/10.1016/0031-9422(80)85120-X, 1980.
Dong, L., Li, Z., and Jia, G.: Archaeal ammonia oxidation plays a part in late Quaternary nitrogen cycling in the South China Sea, Earth Planet. Sci. Lett., 509, 38–46, https://doi.org/10.1016/j.epsl.2018.12.023, 2019.
Douglas, P. M. J., Affek, H. P., Ivany, L. C., Houben, A. J. P., Sijp, W. P., Sluijs, A., Schouten, S., and Pagani, M.: Pronounced zonal heterogeneity in Eocene southern high-latitude sea surface temperatures, P. Natl. Acad. Sci. USA, 111, 6582–6587, 2014.
Eberle, J. J. and Greenwood, D. R.: Life at the top of the greenhouse Eocene world–A review of the Eocene flora and vertebrate fauna from Canada's High Arctic, Geol. Soc. Am. Bull., 124, 3–23, https://doi.org/10.1130/B30571.1, 2012.
Elling, F. J., Könneke, M., Lipp, J. S., Becker, K. W., Gagen, E. J., and Hinrichs, K.-U.: Effects of growth phase on the membrane lipid composition of the thaumarchaeon Nitrosopumilus maritimus and their implications for archaeal lipid distributions in the marine environment, Geochim. Cosmochim. Ac., 141, 579–597, https://doi.org/10.1016/j.gca.2014.07.005, 2014.
Elling, F. J., Könneke, M., Mußmann, M., Greve, A., and Hinrichs, K.-U.: Influence of temperature, pH, and salinity on membrane lipid composition and TEX86 of marine planktonic thaumarchaeal isolates, Geochim. Cosmochim. Ac., 171, 238–255, https://doi.org/10.1016/j.gca.2015.09.004, 2015.
Estes, R. and Hutchinson, J. H.: Eocene Lower Vertebrates from Ellesmere Island, Canadian Arctic Archipelago, Palaeogeogr. Palaeocl., 30, 325–347, 1980.
Evans, D., Sagoo, N., Renema, W., Cotton, L. J., Müller, W., Todd, J. A., Saraswati, P. K., Stassen, P., Ziegler, M., Pearson, P. N., Valdes, P. J., and Affek, H. P.: Eocene greenhouse climate revealed by coupled clumped isotope-Mg/Ca thermometry, P. Natl. Acad. Sci. USA, 115, 1174–1179, https://doi.org/10.1073/pnas.1714744115, 2018.
Frieling, J. and Sluijs, A.: Towards quantitative environmental reconstructions from ancient non-analogue microfossil assemblages: Ecological preferences of Paleocene – Eocene dinoflagellates, Earth-Sci. Rev., 185, 956–973, https://doi.org/10.1016/j.earscirev.2018.08.014, 2018.
Frieling, J., Iakovleva, A. I., Reichart, G. J., Aleksandrova, G. N., Gnibidenko, Z. N., Schouten, S., and Sluijs, A.: Paleocene-Eocene warming and biotic response in the epicontinental West Siberian Sea, Geology, 42, 767–770, 2014.
Frieling, J., Gebhardt, H., Huber, M., Adekeye, O. A., Akande, S. O., Reichart, G.-J., Middelburg, J. J., Schouten, S., and Sluijs, A.: Extreme warmth and heat-stressed plankton in the tropics during the Paleocene-Eocene Thermal Maximum, Sci. Adv., 3, e1600891, https://doi.org/10.1126/sciadv.1600891, 2017.
Greenwood, D. R. and Wing, S. L.: Eocene continental climates and latitudinal temperature gradients, Geology, 23, 1044–1048, 1995.
Greenwood, D. R., Basinger, J. F., and Smith, R. Y.: How wet was the Arctic Eocene rain forest? Estimates of precipitation from Paleogene Arctic macrofloras, Geology, 38, 15–18, https://doi.org/10.1130/G30218.1, 2010.
Harding, I. C., Charles, A. J., Marshall, J. E. A., Pälike, H., Roberts, A. P., Wilson, P. A., Jarvis, E., Thorne, R., Morris, E., Moremon, R., Pearce, R. B., and Akbari, S.: Sea-level and salinity fluctuations during the Paleocene-Eocene thermal maximum in Arctic Spitsbergen, Earth Planet. Sci. Lett., 303, 97–107, 2011.
Heer, O.: Flora fossilis Arctica, Kongliga Swenska Wetenskaps Academiens Handlingar, 4, Stockholm, Sweden, 41 pp., 1869.
Ho, S. L. and Laepple, T.: Flat meridional temperature gradient in the early Eocene in the subsurface rather than surface ocean, Nat. Geosci., 9, 606–610, https://doi.org/10.1038/ngeo2763, 2016.
Ho, S. L., Mollenhauer, G., Fietz, S., Martínez-Garcia, A., Lamy, F., Rueda, G., Schipper, K., Méheust, M., Rosell-Melé, A., Stein, R., and Tiedemann, R.: Appraisal of TEX86 and TEX86L thermometries in subpolar and polar regions, Geochim. Cosmochim. Ac., 131, 213–226, https://doi.org/10.1016/j.gca.2014.01.001, 2014.
Hollis, C. J., Dunkley Jones, T., Anagnostou, E., Bijl, P. K., Cramwinckel, M. J., Cui, Y., Dickens, G. R., Edgar, K. M., Eley, Y., Evans, D., Foster, G. L., Frieling, J., Inglis, G. N., Kennedy, E. M., Kozdon, R., Lauretano, V., Lear, C. H., Littler, K., Lourens, L., Meckler, A. N., Naafs, B. D. A., Pälike, H., Pancost, R. D., Pearson, P. N., Röhl, U., Royer, D. L., Salzmann, U., Schubert, B. A., Seebeck, H., Sluijs, A., Speijer, R. P., Stassen, P., Tierney, J., Tripati, A., Wade, B., Westerhold, T., Witkowski, C., Zachos, J. C., Zhang, Y. G., Huber, M., and Lunt, D. J.: The DeepMIP contribution to PMIP4: methodologies for selection, compilation and analysis of latest Paleocene and early Eocene climate proxy data, incorporating version 0.1 of the DeepMIP database, Geosci. Model Dev., 12, 3149–3206, https://doi.org/10.5194/gmd-12-3149-2019, 2019.
Hopmans, E. C., Schouten, S., Pancost, R. D., van der Meer, M. T. J., and Damsté, J. S. S.: Analysis of intact tetraether lipids in archaeal cell material and sediments by high performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry, Rapid. Commun. Mass. Sp., 14, 585–589, 2000.
Hopmans, E. C., Weijers, J. W. H., Schefuß, E., Herfort, L., Sinninghe Damsté, J. S., and Schouten, S.: A novel proxy for terrestrial organic matter in sediments based on branched and isoprenoid tetraether lipids, Earth Planet. Sci. Lett., 224, 107–116, 2004.
Hopmans, E. C., Schouten, S., and Sinninghe Damsté, J. S.: The effect of improved chromatography on GDGT-based palaeoproxies, Org. Geochem., 93, 1–6, https://doi.org/10.1016/j.orggeochem.2015.12.006, 2016.
Hurley, S. J., Elling, F. J., Könneke, M., Buchwald, C., Wankel, S. D., Santoro, A. E., Lipp, J. S., Hinrichs, K.-U., and Pearson, A.: Influence of ammonia oxidation rate on thaumarchaeal lipid composition and the TEX86 temperature proxy, P. Natl. Acad. Sci. USA, 113, 7762–7767, https://doi.org/10.1073/pnas.1518534113, 2016.
Inglis, G. N., Farnsworth, A., Lunt, D., Foster, G. L., Hollis, C. J., Pagani, M., Jardine, P. E., Pearson, P. N., Markwick, P., Galsworthy, A. M. J., Raynham, L., Taylor, K. W. R., and Pancost, R. D.: Descent toward the Icehouse: Eocene sea surface cooling inferred from GDGT distributions, Paleoceanography, 30, 1000–1020, https://doi.org/10.1002/2014PA002723, 2015.
Inglis, G. N., Farnsworth, A., Collinson, M. E., Carmichael, M. J., Naafs, B. D. A., Lunt, D. J., Valdes, P. J., and Pancost, R. D.: Terrestrial environmental change across the onset of the PETM and the associated impact on biomarker proxies: A cautionary tale, Global Planet. Change, 181, 102991, https://doi.org/10.1016/j.gloplacha.2019.102991, 2019.
Jaeschke, A., Jørgensen, S. L., Bernasconi, S. M., Pedersen, R. B., Thorseth, I. H., and Früh-Green, G. L.: Microbial diversity of Loki's Castle black smokers at the Arctic Mid-Ocean Ridge, Geobiology, 10, 548–561, https://doi.org/10.1111/gbi.12009, 2012
Karner, M. B., DeLong, E. F., and Karl, D. M.: Archaeal dominance in the mesopelagic zone of the Pacific Ocean, Nature, 409, 507–510, https://doi.org/10.1038/35054051, 2001.
Kim, J.-H., Schouten, S., Hopmans, E. C., Donner, B., and Sinninghe Damste, J. S.: Global sediment core-top calibration of the TEX86 paleothermometer in the ocean, Geochim. Cosmochim. Ac., 72, 1154–1173, 2008.
Kim, J.-H., van der Meer, J., Schouten, S., Helmke, P., Willmott, V., Sangiorgi, F., Koç, N., Hopmans, E. C., and Sinninghe Damsté, J. S.: New indices and calibrations derived from the distribution of crenarchaeal isoprenoid tetraether lipids: Implications for past sea surface temperature reconstructions, Geochim. Cosmochim. Ac., 74, 4639–4654, 2010.
Kim, J.-H., Schouten, S., Rodrigo-Gámiz, M., Rampen, S., Marino, G., Huguet, C., Helmke, P., Buscail, R., Hopmans, E. C., Pross, J., Sangiorgi, F., Middelburg, J. B. M., and Sinninghe Damsté, J. S.: Influence of deep-water derived isoprenoid tetraether lipids on the paleothermometer in the Mediterranean Sea, Geochim. Cosmochim. Ac., 150, 125–141, https://doi.org/10.1016/j.gca.2014.11.017, 2015.
Kirkels, F. M. S. A., Ponton, C., Galy, V., West, A. J., Feakins, S. J., and Peterse, F.: From Andes to Amazon: Assessing Branched Tetraether Lipids as Tracers for Soil Organic Carbon in the Madre de Dios River System, J. Geophys. Res.-Biogeo., 125, e2019JG005270, https://doi.org/10.1029/2019jg005270, 2020.
Knies, J., Mann, U., Popp, B. N., Stein, R., and Brumsack, H.-J.: Surface water productivity and paleoceanographic implications in the Cenozoic Arctic Ocean, Paleoceanography, 23, PA1S16, https://doi.org/10.1029/2007pa001455, 2008.
Könneke, M., Bernhard, A. E., de la Torre, J. R., Walker, C. B., Waterbury, J. B., and Stahl, D. A.: Isolation of an autotrophic ammonia-oxidizing marine archaeon, Nature, 437, 543–546, https://doi.org/10.1038/nature03911, 2005.
Liu, X.-L., Summons, R. E., and Hinrichs, K.-U.: Extending the known range of glycerol ether lipids in the environment: structural assignments based on tandem mass spectral fragmentation patterns, Rapid. Commun. Mass. Sp., 26, 2295–2302, https://doi.org/10.1002/rcm.6355, 2012.
Liu, Z., Pagani, M., Zinniker, D., DeConto, R., Huber, M., Brinkhuis, H., Shah, S. R., Leckie, R. M., and Pearson, A.: Global Cooling During the Eocene-Oligocene Climate Transition, Science, 323, 1187–1190, https://doi.org/10.1126/science.1166368, 2009.
Lunt, D. J., Dunkley Jones, T., Heinemann, M., Huber, M., LeGrande, A., Winguth, A., Loptson, C., Marotzke, J., Roberts, C. D., Tindall, J., Valdes, P., and Winguth, C.: A model–data comparison for a multi-model ensemble of early Eocene atmosphere–ocean simulations: EoMIP, Clim. Past, 8, 1717–1736, https://doi.org/10.5194/cp-8-1717-2012, 2012.
März, C., Schnetger, B., and Brumsack, H. J.: Paleoenvironmental implications of Cenozoic sediments from the central Arctic Ocean (IODP Expedition 302) using inorganic geochemistry, Paleoceanography, 25, PA3206, https://doi.org/10.1029/2009pa001860, 2010.
Menzel, D., Hopmans, E. C., Schouten, S., and Sinninghe Damsté, J. S.: Membrane tetraether lipids of planktonic Crenarchaeota in Pliocene sapropels of the eastern Mediterranean Sea, Palaeogeogr. Palaeocl., 239, 1–15, 2006.
Mollenhauer, G., Basse, A., Kim, J.-H., Sinninghe Damsté, J. S., and Fischer, G.: A four-year record of UK′37- and TEX86-derived sea surface temperature estimates from sinking particles in the filamentous upwelling region off Cape Blanc, Mauritania, Deep Sea Res. Pt. I, 97, 67–79, https://doi.org/10.1016/j.dsr.2014.11.015, 2015.
Morales, C., Rogov, M., Wierzbowski, H., Ershova, V., Suan, G., Adatte, T., Föllmi, K. B., Tegelaar, E., Reichart, G.-J., de Lange, G. J., Middelburg, J. J., and van de Schootbrugge, B.: Glendonites track methane seepage in Mesozoic polar seas, Geology, 45, 503–506, https://doi.org/10.1130/g38967.1, 2017.
Müller, R. D., Cannon, J., Qin, X., Watson, R. J., Gurnis, M., Williams, S., Pfaffelmoser, T., Seton, M., Russell, S. H. J., and Zahirovic, S.: GPlates: Building a Virtual Earth Through Deep Time, Geochem., Geophys., Geosys., 19, 2243–2261, https://doi.org/10.1029/2018gc007584, 2018.
Naafs, B. D. A., Inglis, G. N., Zheng, Y., Amesbury, M. J., Biester, H., Bindler, R., Blewett, J., Burrows, M. A., del Castillo Torres, D., Chambers, F. M., Cohen, A. D., Evershed, R. P., Feakins, S. J., Gałka, M., Gallego-Sala, A., Gandois, L., Gray, D. M., Hatcher, P. G., Honorio Coronado, E. N., Hughes, P. D. M., Huguet, A., Könönen, M., Laggoun-Défarge, F., Lähteenoja, O., Lamentowicz, M., Marchant, R., McClymont, E., Pontevedra-Pombal, X., Ponton, C., Pourmand, A., Rizzuti, A. M., Rochefort, L., Schellekens, J., De Vleeschouwer, F., and Pancost, R. D.: Introducing global peat-specific temperature and pH calibrations based on brGDGT bacterial lipids, Geochim. Cosmochim. Ac., 208, 285–301, https://doi.org/10.1016/j.gca.2017.01.038, 2017.
Naafs, B. D. A., McCormick, D., Inglis, G. N., and Pancost, R. D.: Archaeal and bacterial H-GDGTs are abundant in peat and their relative abundance is positively correlated with temperature, Geochim. Cosmochim. Ac., 227, 156–170, https://doi.org/10.1016/j.gca.2018.02.025, 2018a.
Naafs, B. D. A., Rohrssen, M., Inglis, G. N., Lähteenoja, O., Feakins, S. J., Collinson, M. E., Kennedy, E. M., Singh, P. K., Singh, M. P., Lunt, D. J., and Pancost, R. D.: High temperatures in the terrestrial mid-latitudes during the early Palaeogene, Nat. Geosci., 11, 766–771, https://doi.org/10.1038/s41561-018-0199-0, 2018b.
O'Brien, C. L., Robinson, S. A., Pancost, R. D., Sinninghe Damsté, J. S., Schouten, S., Lunt, D. J., Alsenz, H., Bornemann, A., Bottini, C., Brassell, S. C., Farnsworth, A., Forster, A., Huber, B. T., Inglis, G. N., Jenkyns, H. C., Linnert, C., Littler, K., Markwick, P., McAnena, A., Mutterlose, J., Naafs, B. D. A., Püttmann, W., Sluijs, A., van Helmond, N. A. G. M., Vellekoop, J., Wagner, T., and Wrobel, N. E.: Cretaceous sea-surface temperature evolution: Constraints from TEX86 and planktonic foraminiferal oxygen isotopes, Earth-Sci. Rev., 172, 224–247, https://doi.org/10.1016/j.earscirev.2017.07.012, 2017.
O'Regan, M., Moran, K., Sangiorgi, F., Brinkhuis, H., Backman, J., Jakobsson, M., Stickley, C. E., Koc, N., Brumsack, H., Willard, D., Pockalny, R., and Skelton, A.: Mid-Cenozoic Tectonic and Paleoenvironmental setting of the Central Arctic Ocean, Paleoceanography, 23, PA1S20, https://doi.org/10.1029/2007PA001559, 2008.
Pagani, M., Pedentchouk, N., Huber, M., Sluijs, A., Schouten, S., Brinkhuis, H., Sinninghe Damsté, J. S., Dickens, G. R., and Expedition 302 Scientists, T.: Arctic hydrology during global warming at the Palaeocene-Eocene thermal maximum, Nature, 442, 671–675, 2006.
Pancost, R. D., Hopmans, E. C., and Sinninghe Damsté, J. S.: Archaeal lipids in mediterranean cold seeps: Molecular proxies for anaerobic methane oxidation, Geochim. Cosmochim. Ac., 65, 1611–1627, 2001.
Park, E., Hefter, J., Fischer, G., Iversen, M. H., Ramondenc, S., Nöthig, E.-M., and Mollenhauer, G.: Seasonality of archaeal lipid flux and GDGT-based thermometry in sinking particles of high-latitude oceans: Fram Strait (79∘ N) and Antarctic Polar Front (50∘ S), Biogeosciences, 16, 2247–2268, https://doi.org/10.5194/bg-16-2247-2019, 2019.
Pierrehumbert, R. T.: The hydrologic cycle in deep-time climate problems, Nature, 419, 191–198, 2002.
Pitcher, A., Hopmans, E. C., Mosier, A. C., Park, S.-J., Rhee, S.-K., Francis, C. A., Schouten, S., and Sinninghe Damsté, J. S.: Core and Intact Polar Glycerol Dibiphytanyl Glycerol Tetraether Lipids of Ammonia-Oxidizing Archaea Enriched from Marine and Estuarine Sediments, Appl. Environ. Microbiol., 77, 3468–3477, https://doi.org/10.1128/aem.02758-10, 2011a.
Pitcher, A., Wuchter, C., Siedenberg, K., Schouten, S., and Sinninghe Damsté, J. S.: Crenarchaeol tracks winter blooms of ammonia-oxidizing Thaumarchaeota in the coastal North Sea, Limnol. Oceanogr., 56, 2308–2318, https://doi.org/10.4319/lo.2011.56.6.2308, 2011b.
Qin, W., Amin, S. A., Martens-Habbena, W., Walker, C. B., Urakawa, H., Devol, A. H., Ingalls, A. E., Moffett, J. W., Armbrust, E. V., and Stahl, D. A.: Marine ammonia-oxidizing archaeal isolates display obligate mixotrophy and wide ecotypic variation, P. Natl. Acad. Sci. USA, 111, 12504–12509, https://doi.org/10.1073/pnas.1324115111, 2014.
Qin, W., Carlson, L. T., Armbrust, E. V., Devol, A. H., Moffett, J. W., Stahl, D. A., and Ingalls, A. E.: Confounding effects of oxygen and temperature on the TEX86 signature of marine Thaumarchaeota, P. Natl. Acad. Sci. USA, 112, 10979–10984, https://doi.org/10.1073/pnas.1501568112, 2015.
Reichgelt, T., West, C. K., and Greenwood, D. R.: The relation between global palm distribution and climate, Sci. Rep.-UK, 8, 4721, https://doi.org/10.1038/s41598-018-23147-2, 2018.
Richey, J. N. and Tierney, J. E.: GDGT and alkenone flux in the northern Gulf of Mexico: Implications for the TEX86 and UK'37 paleothermometers, Paleoceanography, 31, 1547–1561, https://doi.org/10.1002/2016pa003032, 2016.
Sangiorgi, F., van Soelen, E. E., Spofforth, D. J. A., Pälike, H., Stickley, C. E., St. John, K., Koç, N., Schouten, S., Sinninghe Damsté, J. S., and Brinkhuis, H.: Cyclicity in the middle Eocene central Arctic Ocean sediment record: Orbital forcing and environmental response, Paleoceanography, 23, PA1S08, https://doi.org/10.1029/2007PA001487, 2008.
Schouten, S., Hopmans, E. C., Schefuß, E., and Sinninghe Damsté, J. S.: Distributional variations in marine crenarchaeotal membrane lipids: a new tool for reconstructing ancient sea water temperatures?, Earth Planet. Sci. Lett., 204, 265–274, 2002.
Schouten, S., Hopmans, E. C., Forster, A., Breugel, Y. V., Kuypers, M. M. M., and Sinninghe Damsté, J. S.: Extremely high sea-surface temperatures at low latitudes during the middle Cretaceous as revealed by archaeal membrane lipids, Geology, 31, 1069–1072, 2003.
Schouten, S., Forster, A., Panoto, F. E., and Sinninghe Damste, J. S.: Towards calibration of the TEX86 palaeothermometer for tropical sea surface temperatures in ancient greenhouse worlds, Org. Geochem., 38, 1537–1546, 2007.
Schouten, S., Baas, M., Hopmans, E. C., and Sinninghe Damsté, J. S.: An unusual isoprenoid tetraether lipid in marine and lacustrine sediments, Org. Geochem., 39, 1033–1038, 2008.
Schouten, S., Hopmans, E. C., van der Meer, J., Mets, A., Bard, E., Bianchi, T. S., Diefendorf, A., Escala, M., Freeman, K. H., Furukawa, Y., Ingalls, C. H. a. A., Ménot-Combes, G., Nederbragt, A. J., Oba, M., Pearson, A., Pearson, E. J., Rosell-Melé, A., Schaeffer, P., Shah, S. R., Shanahan, T. M., Smith, R. W., Smittenberg, R., Talbot, H. M., Uchida, M., Mooy, B. A. S. V., Yamamoto, M., Zhang, Z., and Sinninghe Damsté, J. S.: An interlaboratory study of TEX86 and BIT analysis using high-performance liquid chromatography–mass spectrometry, Geochem. Geophys. Geosys., 10, Q03012, https://doi.org/10.1029/2008GC002221, 2009.
Schouten, S., Hopmans, E. C., and Sinninghe Damsté, J. S.: The organic geochemistry of glycerol dialkyl glycerol tetraether lipids: A review, Org. Geochem., 54, 19–61, https://doi.org/10.1016/j.orggeochem.2012.09.006, 2013.
Schweitzer, H.-J.: Environment and climate in the early Tertiary of Spitsbergen, Palaeogeogr. Palaeocl., 30, 297–311, 1980.
Seton, M., Müller, R. D., Zahirovic, S., Gaina, C., Torsvik, T., Shephard, G., Talsma, A., Gurnis, M., Turner, M., Maus, S., and Chandler, M.: Global continental and ocean basin reconstructions since 200Ma, Earth-Sci. Rev., 113, 212–270, https://doi.org/10.1016/j.earscirev.2012.03.002, 2012.
Shah, S. R., Mollenhauer, G., Ohkouchi, N., Eglinton, T. I., and Pearson, A.: Origins of archaeal tetraether lipids in sediments: Insights from radiocarbon analysis, Geochim. Cosmochim. Ac., 72, 4577–4594, https://doi.org/10.1016/j.gca.2008.06.021, 2008.
Sinninghe Damsté, J. S., Wakeham, S. G., Kohnen, M. E. L., Hayes, J. M., and de Leeuw, J. W.: A 6,000-year sedimentary molecular record of chemocline excursions in the Black Sea, Nature, 362, 827–829, 1993.
Sinninghe Damsté, J. S., Rijpstra, W. I. C., Hopmans, E. C., Weijers, J. W. H., Foesel, B. U., Overmann, J., and Dedysh, S. N.: 13,16-Dimethyl Octacosanedioic Acid (iso-Diabolic Acid), a Common Membrane-Spanning Lipid of Acidobacteria Subdivisions 1 and 3, Appl. Environ. Microbiol., 77, 4147–4154, https://doi.org/10.1128/aem.00466-11, 2011.
Sinninghe Damsté, J. S.: Spatial heterogeneity of sources of branched tetraethers in shelf systems: The geochemistry of tetraethers in the Berau River delta (Kalimantan, Indonesia), Geochim. Cosmochim. Ac., 186, 13–31, https://doi.org/10.1016/j.gca.2016.04.033, 2016.
Sinninghe Damsté, J. S., Rijpstra, W. I. C., Foesel, B. U., Huber, K. J., Overmann, J., Nakagawa, S., Kim, J. J., Dunfield, P. F., Dedysh, S. N., and Villanueva, L.: An overview of the occurrence of ether- and ester-linked iso-diabolic acid membrane lipids in microbial cultures of the Acidobacteria: Implications for brGDGT paleoproxies for temperature and pH, Org. Geochem., 124, 63–76, https://doi.org/10.1016/j.orggeochem.2018.07.006, 2018a.
Sinninghe Damsté, J. S., Rijpstra, W. I. C., Hopmans, E. C., den Uijl, M. J., Weijers, J. W. H., and Schouten, S.: The enigmatic structure of the crenarchaeol isomer, Org. Geochem., 124, 22–28, https://doi.org/10.1016/j.orggeochem.2018.06.005, 2018b.
Sluijs, A.: GDGT and GMGT data, PANGAEA, https://doi.org/10.1594/PANGAEA.924854, 2020.
Sluijs, A. and Dickens, G. R.: Assessing offsets between the δ13C of sedimentary components and the global exogenic carbon pool across Early Paleogene carbon cycle perturbations, Global Biogeochem. Cy., 26, GB4005, https://doi.org/10.1029/2011GB004224, 2012.
Sluijs, A., Schouten, S., Pagani, M., Woltering, M., Brinkhuis, H., Sinninghe Damsté, J. S., Dickens, G. R., Huber, M., Reichart, G.-J., Stein, R., Matthiessen, J., Lourens, L. J., Pedentchouk, N., Backman, J., Moran, K., and The Expedition 302 Scientists: Subtropical Arctic Ocean temperatures during the Palaeocene/Eocene thermal maximum, Nature, 441, 610–613, 2006.
Sluijs, A., Brinkhuis, H., Crouch, E. M., John, C. M., Handley, L., Munsterman, D., Bohaty, S., M., Zachos, J. C., Reichart, G.-J., Schouten, S., Pancost, R. D., Sinninghe Damsté, J. S., Welters, N. L. D., Lotter, A. F., and Dickens, G. R.: Eustatic variations during the Paleocene-Eocene greenhouse world, Paleoceanography, 23, PA4216, https://doi.org/10.1029/2008PA001615, 2008a.
Sluijs, A., Röhl, U., Schouten, S., Brumsack, H.-J., Sangiorgi, F., Sinninghe Damsté, J. S., and Brinkhuis, H.: Arctic late Paleocene–early Eocene paleoenvironments with special emphasis on the Paleocene-Eocene thermal maximum (Lomonosov Ridge, Integrated Ocean Drilling Program Expedition 302), Paleoceanography, 23, PA1S11, https://doi.org/10.1029/2007PA001495, 2008b.
Sluijs, A., Schouten, S., Donders, T. H., Schoon, P. L., Röhl, U., Reichart, G. J., Sangiorgi, F., Kim, J.-H., Sinninghe Damsté, J. S., and Brinkhuis, H.: Warm and Wet Conditions in the Arctic Region during Eocene Thermal Maximum 2, Nat. Geosci., 2, 777–780, 2009.
Sollich, M., Yoshinaga, M. Y., Häusler, S., Price, R. E., Hinrichs, K.-U., and Bühring, S. I.: Heat Stress Dictates Microbial Lipid Composition along a Thermal Gradient in Marine Sediments, Front. Microbiol., 8, 1550, https://doi.org/10.3389/fmicb.2017.01550, 2017.
Spielhagen, R. F. and Tripati, A.: Evidence from Svalbard for near-freezing temperatures and climate oscillations in the Arctic during the Paleocene and Eocene, Palaeogeogr. Palaeocl., 278, 48–56, https://doi.org/10.1016/j.palaeo.2009.04.012, 2009.
Stein, R.: Upper Cretaceous/lower Tertiary black shales near the North Pole: Organic-carbon origin and source-rock potential, Mar. Petrol. Geol., 24, 67–73, 2007.
Stein, R., Boucsein, B., and Meyer, H.: Anoxia and high primary production in the Paleogene central Arctic Ocean: First detailed records from Lomonosov Ridge, Geophys. Res. Lett., 33, L18606, https://doi.org/10.1029/2006GL026776, 2006.
Suan, G., Popescu, S.-M., Yoon, D., Baudin, F., Suc, J.-P., Schnyder, J., Labrousse, L., Fauquette, S., Piepjohn, K., and Sobolev, N. N.: Subtropical climate conditions and mangrove growth in Arctic Siberia during the early Eocene, Geology, 45, 539–542, https://doi.org/10.1130/g38547.1, 2017.
Taylor, K. W. R., Huber, M., Hollis, C. J., Hernandez-Sanchez, M. T., and Pancost, R. D.: Re-evaluating modern and Palaeogene GDGT distributions: Implications for SST reconstructions, Global Planet. Change, 108, 158–174, https://doi.org/10.1016/j.gloplacha.2013.06.011, 2013.
Teichert, B. M. A. and Luppold, F. W.: Glendonites from an Early Jurassic methane seep – Climate or methane indicators?, Palaeogeogr. Palaeocl., 390, 81–93, https://doi.org/10.1016/j.palaeo.2013.03.001, 2013.
Tierney, J. E. and Tingley, M. P.: A Bayesian, spatially-varying calibration model for the TEX86 proxy, Geochim. Cosmochim. Ac., 127, 83–106, https://doi.org/10.1016/j.gca.2013.11.026, 2014.
Tierney, J. E., Sinninghe Damsté, J. S., Pancost, R. D., Sluijs, A., and Zachos, J. C.: Eocene temperature gradients, Nat. Geosci., 10, 538–539, https://doi.org/10.1038/ngeo2997, 2017.
Torsvik, T. H., Van der Voo, R., Preeden, U., Mac Niocaill, C., Steinberger, B., Doubrovine, P. V., van Hinsbergen, D. J. J., Domeier, M., Gaina, C., Tohver, E., Meert, J. G., McCausland, P. J. A., and Cocks, L. R. M.: Phanerozoic polar wander, palaeogeography and dynamics, Earth-Sci. Rev., 114, 325–368, https://doi.org/10.1016/j.earscirev.2012.06.007, 2012.
Trommer, G., Siccha, M., van der Meer, M. T. J., Schouten, S., Sinninghe Damsté, J. S., Schulz, H., Hemleben, C., and Kucera, M.: Distribution of Crenarchaeota tetraether membrane lipids in surface sediments from the Red Sea, Org. Geochem., 40, 724–731, https://doi.org/10.1016/j.orggeochem.2009.03.001, 2009.
Uhl, D., Traiser, C., Griesser, U., and Denk, T.: Fossil leaves as palaeoclimate proxies in the Palaeogene of Spitsbergen (Svalbard), Acta Palaeobotanica, 47, 89–107, 2007.
Van der Burgh, J.: Some palms in the Miocene of the lower Rhenish Plain, Rev. Palaebot. Palyno., 40, 359–374, 1984.
Van Hinsbergen, D. J. J., de Groot, L. V., van Schaik, S. J., Spakman, W., Bijl, P. K., Sluijs, A., Langereis, C. G., and Brinkhuis, H.: A Paleolatitude Calculator for Paleoclimate Studies, Plos One, 10, e0126946, https://doi.org/10.1371/journal.pone.0126946, 2015.
Wakeham, S. G., Amann, R., Freeman, K. H., Hopmans, E. C., Jörgensen, B. B., Putnam, I. F., Schouten, S., Sinninghe Damsté, J. S., Talbot, H. M., and Woebken, D.: Microbial ecology of the stratified water column of the Black Sea as revealed by a comprehensive biomarker stud, Org. Geochem., 38, 2070–2097, 2007.
Weijers, J. W. H., Schouten, S., Spaargaren, O. C., and Sinninghe Damsté, J. S.: Occurrence and distribution of tetraether membrane lipids in soils: Implications for the use of the TEX86 proxy and the BIT index, Org. Geochem., 37, 1680–1693, 2006.
Weijers, J. W. H., Schouten, S., Sluijs, A., Brinkhuis, H., and Sinninghe Damsté, J. S.: Warm arctic continents during the Palaeocene-Eocene thermal maximum, Earth Planet. Sci. Lett., 261, 230–238, 2007a.
Weijers, J. W. H., Schouten, S., van den Donker, J. C., Hopmans, E. C., and Sinninghe Damsté, J. S.: Environmental controls on bacterial tetraether membrane lipid distribution in soils, Geochim. Cosmochim. Ac., 71, 703–713, 2007b.
Weijers, J. W. H., Lim, K. L. H., Aquilina, A., Sinninghe Damsté, J. S., and Pancost, R. D.: Biogeochemical controls on glycerol dialkyl glycerol tetraether lipid distributions in sediments characterized by diffusive methane flux, Geochem. Geophys. Geosys., 12, Q10010, https://doi.org/10.1029/2011GC003724, 2011.
Willard, D. A., Donders, T. H., Reichgelt, T., Greenwood, D. R., Sangiorgi, F., Peterse, F., Nierop, K. G. J., Frieling, J., Schouten, S., and Sluijs, A.: Arctic vegetation, temperature, and hydrology during Early Eocene transient global warming events, Global Planet. Change, 178, 139–152, https://doi.org/10.1016/j.gloplacha.2019.04.012, 2019.
Wuchter, C., Schouten, S., Coolen, M. J. L., and Sinninghe Damsté, J. S.: Temperature-dependent variation in the distribution of tetraether membrane lipids of marine Crenarchaeota: Implications for TEX86 paleothermometry, Paleoceanography, 19, PA402, https://doi.org/10.1029/2004PA001041, 2004.
Wuchter, C., Schouten, S., Wakeham, S. G., and Sinninghe Damste, J. S.: Temporal and spatial variation in tetraether membrane lipids of marine Crenarchaeota in particulate organic matter: Implications for TEX86 paleothermometry, Paleoceanography, 20, PA3013, https://doi.org/10.1029/2004PA001110, 2005.
Wuchter, C., Abbas, B., Coolen, M. J. L., Herfort, L., van Bleijswijk, J., Timmers, P., Strous, M., Teira, E., Herndl, G. J., Middelburg, J. J., Schouten, S., and Damste, J. S. S.: Archaeal nitrification in the ocean, P. Natl. Acad. Sci. USA, 103, 12317–12322, https://doi.org/10.1073/pnas.0600756103, 2006a.
Wuchter, C., Schouten, S., Wakeham, S. G., and Sinninghe Damsté, J. S.: Archaeal tetraether membrane lipid fluxes in the northeastern Pacific and the Arabian Sea: Implications for TEX86 paleothermometry, Paleoceanography, 21, PA4208, 2006b.
Xie, S., Liu, X.-L., Schubotz, F., Wakeham, S. G., and Hinrichs, K.-U.: Distribution of glycerol ether lipids in the oxygen minimum zone of the Eastern Tropical North Pacific Ocean, Org. Geochem., 71, 60–71, https://doi.org/10.1016/j.orggeochem.2014.04.006, 2014.
Yamamoto, M., Shimamoto, A., Fukuhara, T., Tanaka, Y., and Ishizaka, J.: Glycerol dialkyl glycerol tetraethers and TEX86 index in sinking particles in the western North Pacific, Org. Geochem., 53, 52–62, https://doi.org/10.1016/j.orggeochem.2012.04.010, 2012.
Zeng, Z., Liu, X.-L., Farley, K. R., Wei, J. H., Metcalf, W. W., Summons, R. E., and Welander, P. V.: GDGT cyclization proteins identify the dominant archaeal sources of tetraether lipids in the ocean, P. Natl. Acad. Sci. USA, 116, 22505–22511, https://doi.org/10.1073/pnas.1909306116, 2019.
Zhang, Y. G. and Liu, X.: Export Depth of the TEX86 Signal, Paleoceanogr. Paleoclimatol., 33, 666–671, https://doi.org/10.1029/2018pa003337, 2018.
Zhang, Y. G., Zhang, C. L., Liu, X.-L., Li, L., Hinrichs, K.-U., and Noakes, J. E.: Methane Index: A tetraether archaeal lipid biomarker indicator for detecting the instability of marine gas hydrates, Earth Planet. Sci. Lett., 307, 525–534, https://doi.org/10.1016/j.epsl.2011.05.031, 2011.
Zhang, Y. G., Pagani, M., and Wang, Z.: Ring Index: A new strategy to evaluate the integrity of TEX86 paleothermometry, Paleoceanography, 31, 220–232, https://doi.org/10.1002/2015PA002848, 2016.
Zhu, J., Poulsen, C. J., and Tierney, J. E.: Simulation of Eocene extreme warmth and high climate sensitivity through cloud feedbacks, Sci. Adv., 5, eaax1874, https://doi.org/10.1126/sciadv.aax1874, 2019.