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<article xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:oasis="http://docs.oasis-open.org/ns/oasis-exchange/table" xml:lang="en" dtd-version="3.0">
  <front>
    <journal-meta><journal-id journal-id-type="publisher">CP</journal-id><journal-title-group>
    <journal-title>Climate of the Past</journal-title>
    <abbrev-journal-title abbrev-type="publisher">CP</abbrev-journal-title><abbrev-journal-title abbrev-type="nlm-ta">Clim. Past</abbrev-journal-title>
  </journal-title-group><issn pub-type="epub">1814-9332</issn><publisher>
    <publisher-name>Copernicus Publications</publisher-name>
    <publisher-loc>Göttingen, Germany</publisher-loc>
  </publisher></journal-meta>
    <article-meta>
      <article-id pub-id-type="doi">10.5194/cp-17-1051-2021</article-id><title-group><article-title>The speleothem oxygen record as a proxy for thermal or moisture changes: a case study of multiproxy records from MIS 5–MIS 6 speleothems from the Demänová Cave system</article-title><alt-title>The speleothem oxygen record as a proxy for thermal or moisture changes</alt-title>
      </title-group><?xmltex \runningtitle{The speleothem oxygen record as a proxy for thermal or moisture changes}?><?xmltex \runningauthor{J.~Pawlak}?>
      <contrib-group>
        <contrib contrib-type="author" corresp="yes">
          <name><surname>Pawlak</surname><given-names>Jacek</given-names></name>
          <email>dzeq@twarda.pan.pl</email>
        </contrib>
        <aff id="aff1"><institution>Institute of Geological Sciences, Polish Academy of Sciences, Warsaw 00-818, Poland</institution>
        </aff>
      </contrib-group>
      <author-notes><corresp id="corr1">Jacek Pawlak (dzeq@twarda.pan.pl)</corresp></author-notes><pub-date><day>18</day><month>May</month><year>2021</year></pub-date>
      
      <volume>17</volume>
      <issue>3</issue>
      <fpage>1051</fpage><lpage>1064</lpage>
      <history>
        <date date-type="received"><day>23</day><month>September</month><year>2020</year></date>
           <date date-type="accepted"><day>13</day><month>April</month><year>2021</year></date>
           <date date-type="rev-recd"><day>11</day><month>April</month><year>2021</year></date>
           <date date-type="rev-request"><day>26</day><month>October</month><year>2020</year></date>
      </history>
      <permissions>
        <copyright-statement>Copyright: © 2021 Jacek Pawlak</copyright-statement>
        <copyright-year>2021</copyright-year>
      <license license-type="open-access"><license-p>This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this licence, visit <ext-link ext-link-type="uri" xlink:href="https://creativecommons.org/licenses/by/4.0/">https://creativecommons.org/licenses/by/4.0/</ext-link></license-p></license></permissions><self-uri xlink:href="https://cp.copernicus.org/articles/17/1051/2021/cp-17-1051-2021.html">This article is available from https://cp.copernicus.org/articles/17/1051/2021/cp-17-1051-2021.html</self-uri><self-uri xlink:href="https://cp.copernicus.org/articles/17/1051/2021/cp-17-1051-2021.pdf">The full text article is available as a PDF file from https://cp.copernicus.org/articles/17/1051/2021/cp-17-1051-2021.pdf</self-uri>
      <abstract><title>Abstract</title>
    <p id="d1e78">Speleothems are an important source of palaeoclimatic information about the terrestrial environment. The basic advantages of speleothems are their
high preservation potential, the possibility of precise dating using the uranium-series (U-series) method, and many different proxies, such as stable isotopes, trace
elements, and microfabrics, which can be interpreted in terms of palaeoclimatic conditions. Currently, central Europe is located in a transitional
climate zone under the influence of both oceanic and continental climates. However, in the past, the region could have been under a stronger
continental climate influence during cold glacial episodes or a stronger oceanic climate influence during wetter interglacial episodes. Long-term
speleothem records can add new beneficial data about past climate changes in the region. The multiproxy record of the JS9 stalagmite, collected in the
Demänová Cave system (Slovakia), represents a ca. 60 <inline-formula><mml:math id="M1" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">kyr</mml:mi></mml:mrow></mml:math></inline-formula> period (143–83 <inline-formula><mml:math id="M2" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>). A multiproxy interpretation of the JS9 record
shows that long-term <inline-formula><mml:math id="M3" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> trends can be interpreted as global/regional temperature changes, whereas short-term <inline-formula><mml:math id="M4" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula>
signals reflect changes in humidity. In contrast to the records from the Alps and the northern Tatra Mountains, the <inline-formula><mml:math id="M5" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> record of
speleothem JS9 shows instantaneous decreasing episodes during Termination II. This indicates that the Carpathian Belt was an important climatic
barrier at that time.</p>
  </abstract>
    </article-meta>
  </front>
<body>
      

<sec id="Ch1.S1" sec-type="intro">
  <label>1</label><title>Introduction</title>
      <p id="d1e151">Speleothems are important palaeoenvironmental archives (Lachniet, 2009; Fairchild and Treble, 2009; Fairchild and Baker, 2012; Koltai et al., 2018;
Kern et al., 2019). Many different palaeoclimatic proxies are currently being studied in speleothems, such as stable isotope compositions, trace
element contents, calcite microfabrics and the isotopic compositions of water from speleothem inclusions (Fairchild and Treble, 2009; Wong and Breecker,
2015; Frisia, 2015; Demény et al., 2017; Baker et al., 2019). Nevertheless, the <inline-formula><mml:math id="M6" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> proxy is still the most commonly used for
regional and global comparisons (Lisiecki and Raymo, 2005; McDermott et al., 2011; Govin et al., 2015). Therefore, understanding which climatic factor
has the strongest influence on <inline-formula><mml:math id="M7" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> composition at a given site is a crucial problem (Lachniet, 2009).</p>
      <p id="d1e184">Basically, the <inline-formula><mml:math id="M8" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value of speleothems reflects the oxygen isotopic composition of precipitation. The isotopic composition of
precipitation depends on global factors, such as the mean isotopic composition of ocean surface water. On a long-term scale, the global volume of
glacial ice has an influence on the <inline-formula><mml:math id="M9" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value of ocean surface water (Dansgaard, 1964). Currently, the Atlantic Ocean is the main
source of vapour for precipitation in Europe. Other potential sources are the Mediterranean Sea, the Black Sea and the Nordic Seas (Ionita, 2014). Water from
the Mediterranean Sea surface is enriched in <inline-formula><mml:math id="M10" display="inline"><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:math></inline-formula> compared with water from the Atlantic Ocean. During glaciations, the Fennoscandian ice sheet
(which is enriched in <inline-formula><mml:math id="M11" display="inline"><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">16</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:math></inline-formula>) affects atmospheric circulation in central Europe and, thus, the isotopic composition of meteoric water (Bianchi
and McCave 1999; Elmore et al., 2015). During deglaciations, cold meltwater can slow down the circulation of Atlantic currents. The consequence of such
a situation for central Europe may be the limited influence of the Atlantic Ocean and the stronger influence of enriched <inline-formula><mml:math id="M12" display="inline"><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:math></inline-formula> moisture
transported from the Black Sea and Mediterranean region (Celle-Jeanton et al., 2001). The other regional factor shaping <inline-formula><mml:math id="M13" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> in
Europe is the continental effect (McDermott<?pagebreak page1052?> et al., 2011). Finally, the isotopic composition of rainwater at the precipitation site is modified by
local factors such as altitude, amount effect and local temperature (Drysdale et al., 2005; Moseley et al., 2015).</p>
      <p id="d1e269">The isotopic composition of dripping waters can also be modified inside the soil and epikarst zone by evaporation and prior calcite precipitation
(PCP; Baker et al., 2019). However, PCP affects the <inline-formula><mml:math id="M14" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value of dissolved <inline-formula><mml:math id="M15" display="inline"><mml:mrow class="chem"><mml:msub><mml:mi mathvariant="normal">CO</mml:mi><mml:mn mathvariant="normal">2</mml:mn></mml:msub></mml:mrow></mml:math></inline-formula> more than the <inline-formula><mml:math id="M16" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value. In a cave
environment, the isotopic fractionation between the dripping water and crystallising calcite depends on the cave air temperature. The cave air
temperature usually reflects the mean annual temperature in the near-cave area. Additionally, the isotopic composition of the crystallising calcite
can be altered by kinetic effects when the relative humidity of the cave air is less than 100 % (Dorale and Liu 2009).</p>
      <p id="d1e313">Recently, more than a dozen speleothem records of the last interglacial age have been obtained from the European continent (Meyer et al., 2008;
Couchoud et al., 2009; Moseley et al., 2015; Vansteenberge et al., 2016; Demény et al., 2017; Pawlak et al., 2019, 2020). The temperature, amount
of precipitation at the cave site and changes in the main source of vapour for precipitation are considered to be the main factors determining the
<inline-formula><mml:math id="M17" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value of precipitated calcite. However, for records from the Alps and central Europe, temperature appears to be a more important
factor (Moseley et al., 2015; Kern et al., 2019; Pawlak et al., 2019, 2020; Comas-Bru et al., 2020). This result is consistent with the observations
made by Różański et al. (1993) which showed that the isotopic composition of rainfall in temperate regions of Europe mainly depends on local
temperature. However, distinguishing which factor is the most dominant is not always a simple task. For example, coastal sites are more influenced by
the amount of precipitation (Couchoud et al., 2009; Vansteenberge et al., 2016).</p>
      <p id="d1e332">Unlike most European records, the records from the Middle East appear to be influenced by more factors, such as the amount of precipitation, the
temperature and changes in the main source of vapour for precipitation (the source effect; Bar-Matthews et al., 2003; Nehme et al., 2015). These
factors can be associated with changes in the prevailing circulation patterns, the impact of evaporation on the Mediterranean Sea surface
<inline-formula><mml:math id="M18" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> and the lower amplitude of long-term mean annual temperature changes during the last interglacial period at lower latitudes
(Rybak et al., 2018).</p>
      <p id="d1e350">Currently, Slovakia is influenced by two main climate types: a boreal and fully humid climate with warm summers (Dfb) in the east, and a warm temperate
and fully humid climate (Cfb) in the west (Kottek et al., 2006). However, in the past, the local climate could have been more continental during the
colder and drier glacial periods and more transitional during the warmer interglacial periods (Feurdean et al., 2014). The new long-term speleothem
records can add beneficial data about past climate changes in this region. This paper presents an approximately 60 <inline-formula><mml:math id="M19" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">kyr</mml:mi></mml:mrow></mml:math></inline-formula> long multiproxy record
(<inline-formula><mml:math id="M20" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula>, <inline-formula><mml:math id="M21" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula>, Mg, Sr, Ba, Na, P, Fe, Mn and Si) of a Marine Isotope Stage (MIS) 5–6 age stalagmite collected in the Demänová
Cave system, Slovakia. The interpretation of these proxies focuses on distinguishing dry continental climate phases from more wet transitional
climate episodes. Additionally, the interpretation of stable isotopic compositions and trace element contents helps to distinguish which factor had
the strongest influence on the shape of the <inline-formula><mml:math id="M22" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> record: the local temperature, the humidity or the source effect.</p>
</sec>
<sec id="Ch1.S2">
  <label>2</label><title>The study setting</title>
      <p id="d1e414">The Demänová Cave system (DCS) is located in the Low Tatras (Fig. 1a), Western Carpathians, Slovakia (49.00<inline-formula><mml:math id="M23" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula> N, 19.58<inline-formula><mml:math id="M24" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula> E;
837 <inline-formula><mml:math id="M25" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">m</mml:mi><mml:mspace width="0.125em" linebreak="nobreak"/><mml:mi mathvariant="normal">a</mml:mi><mml:mo>.</mml:mo><mml:mi mathvariant="normal">s</mml:mi><mml:mo>.</mml:mo><mml:mi mathvariant="normal">l</mml:mi><mml:mo>.</mml:mo></mml:mrow></mml:math></inline-formula>). The DCS is 41.4 <inline-formula><mml:math id="M26" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">km</mml:mi></mml:mrow></mml:math></inline-formula> (Fig. 1b) long, and its denivelation is 196 <inline-formula><mml:math id="M27" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">m</mml:mi></mml:mrow></mml:math></inline-formula> (Herich, 2017). The DCS
includes 10 caves that are connected to each other. Sample JS9 was collected in the Demänovská Slobody Cave (Fig. 1b). The DCS has nine cave levels that
can be correlated with fluvial terraces of the Demänovká Stream according to Droppa (1966, 1972). The cave system was formed by fluvial processes and the
cave corridors were formed by allochthonous sinking streams during the late Pliocene (Bella, 1993). The host rocks of the DCS are Middle Triassic
carbonate rocks, primarily the Gutenstein and Annaberg limestones (early Anisian), organodetritic limestones (late Anisian), and Ramsau dolomite
(Ladinian) (Droppa, 1957; Gaál, 2016; Gaál and Michalík, 2017).</p>

      <?xmltex \floatpos{t}?><fig id="Ch1.F1"><?xmltex \currentcnt{1}?><?xmltex \def\figurename{Figure}?><label>Figure 1</label><caption><p id="d1e474"><bold>(a)</bold> The location of the Demänová Cave system. <bold>(b)</bold> A map of the Demänová Cave system. The numbers used in the panel legends refer to the following: 1 – the Magurska Cave site, 2 – the Baradla Cave site, 3 – the Tatra Mountains, 4 – the Low Tatras, 5 – the Cfb climate zone, 6 – the Dfb climate zone, 7 – the sites with cave temperature monitoring and 8 – the sample collection site.</p></caption>
        <?xmltex \igopts{width=139.418504pt}?><graphic xlink:href="https://cp.copernicus.org/articles/17/1051/2021/cp-17-1051-2021-f01.png"/>

      </fig>

      <p id="d1e488">The DCS is located in a transitional climate zone between oceanic and continental climates (Sotak and Borsanyi, 2004; Kottek et al., 2006). There are
two meteorological stations near the DCS: the first is located in the township of Liptovský Mikuláš close to the Demänovská valley entrance
(49.07<inline-formula><mml:math id="M28" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula> N, 19.61<inline-formula><mml:math id="M29" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula> E; 570 <inline-formula><mml:math id="M30" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">m</mml:mi><mml:mspace linebreak="nobreak" width="0.125em"/><mml:mi mathvariant="normal">a</mml:mi><mml:mo>.</mml:mo><mml:mi mathvariant="normal">s</mml:mi><mml:mo>.</mml:mo><mml:mi mathvariant="normal">l</mml:mi><mml:mo>.</mml:mo></mml:mrow></mml:math></inline-formula>), and the second is located on Chopok peak under the influence of a cold mountain climate
(48.94<inline-formula><mml:math id="M31" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula> N, 19.59<inline-formula><mml:math id="M32" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula> E; 2008 <inline-formula><mml:math id="M33" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">m</mml:mi><mml:mspace linebreak="nobreak" width="0.125em"/><mml:mi mathvariant="normal">a</mml:mi><mml:mo>.</mml:mo><mml:mi mathvariant="normal">s</mml:mi><mml:mo>.</mml:mo><mml:mi mathvariant="normal">l</mml:mi><mml:mo>.</mml:mo></mml:mrow></mml:math></inline-formula>). Chopok peak has a colder and wetter climate (mean annual temperature of
<inline-formula><mml:math id="M34" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>0.1 <inline-formula><mml:math id="M35" display="inline"><mml:mrow class="unit"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:math></inline-formula>; mean annual precipitation of 1325 <inline-formula><mml:math id="M36" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">mm</mml:mi></mml:mrow></mml:math></inline-formula>), whereas Liptovský Mikuláš has a warmer and drier climate
(mean annual temperature of <inline-formula><mml:math id="M37" display="inline"><mml:mo>+</mml:mo></mml:math></inline-formula>6.9 <inline-formula><mml:math id="M38" display="inline"><mml:mrow class="unit"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:math></inline-formula>; mean annual precipitation of 537 <inline-formula><mml:math id="M39" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">mm</mml:mi></mml:mrow></mml:math></inline-formula>). The local climate has strong seasonality: the
coldest and driest months are January and February, whereas the warmest months are July and August; the greatest amount of precipitation is usually noted
in June and July. Despite the high altitude and thermal gradient along the valley, the mean annual <inline-formula><mml:math id="M40" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value of precipitation at
Chopok peak (<inline-formula><mml:math id="M41" display="inline"><mml:mo lspace="0mm">-</mml:mo></mml:math></inline-formula>10.43 ‰) is similar to the value in Liptovský Mikuláš (<inline-formula><mml:math id="M42" display="inline"><mml:mo lspace="0mm">-</mml:mo></mml:math></inline-formula>10.92 ‰) (Holko et al., 2012). The average
cave temperature measured in 2015–2016 at several sites in the DCS was 6.3 <inline-formula><mml:math id="M43" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.6 <inline-formula><mml:math id="M44" display="inline"><mml:mrow class="unit"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:math></inline-formula> (Hercman et al., 2020).</p>
      <p id="d1e674">Based on a large number of uranium-series (U-series) ages, several stages of speleothem crystallisation, which<?pagebreak page1053?> developed chiefly in the warmer periods of the
Pleistocene and in the Holocene, were distinguished in the DCS (e.g. Hercman et al., 1997, 2020; Hercman, 2000; Bella et al., 2021). Field
observations along with a chemical study of underground water in caves (Motyka et al., 2005) suggest that many speleothems still grow in the
DCS. Sample JS9 was collected in the northern part of the Demänovská Slobody Cave (Fig. 1b).</p><?xmltex \hack{\newpage}?>
</sec>
<sec id="Ch1.S3">
  <label>3</label><title>Methods</title>
<sec id="Ch1.S3.SS1">
  <label>3.1</label><title>Petrography</title>
      <p id="d1e693">The entire JS9 stalagmite profile was analysed by a Nikon Eclipse LV100POL microscope, including microfabric structures, the appearance of calcite
crystals, potential discontinuities and porosity. The analysis of speleothem microfabrics and microfabric log construction was based on the
methodology proposed by Frisia (2015). The microscopic analyses were performed at the Institute of Geological Sciences at the Polish Academy of
Sciences (Warsaw, Poland).</p>
</sec>
<sec id="Ch1.S3.SS2">
  <label>3.2</label><title>U-series and age–depth model</title>
      <p id="d1e704">A total of 10 calcite samples (0.1–0.5 <inline-formula><mml:math id="M45" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">g</mml:mi></mml:mrow></mml:math></inline-formula>) were collected by drilling the JS9 speleothem through its growth axes. The samples were drilled as thin as
possible and had an average thickness of 2.5 <inline-formula><mml:math id="M46" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.2 <inline-formula><mml:math id="M47" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">mm</mml:mi></mml:mrow></mml:math></inline-formula>. The chemical preparation of the samples was performed at the Uranium-Series Laboratory of the Institute of Geological Sciences, Polish Academy of Sciences (Warsaw, Poland). In order to control the efficiency of the chemical procedure, a
spike (<inline-formula><mml:math id="M48" display="inline"><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">233</mml:mn></mml:msup><mml:mi mathvariant="normal">U</mml:mi></mml:mrow></mml:math></inline-formula>, <inline-formula><mml:math id="M49" display="inline"><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">236</mml:mn></mml:msup><mml:mi mathvariant="normal">U</mml:mi></mml:mrow></mml:math></inline-formula> and <inline-formula><mml:math id="M50" display="inline"><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">229</mml:mn></mml:msup><mml:mi mathvariant="normal">Th</mml:mi></mml:mrow></mml:math></inline-formula> mixture) was initially added to the samples. In the first step of the chemical
procedure, the samples were heated to decompose potential organic matter. The samples were then dissolved in nitric acid. Finally, uranium and
thorium were separated from the solution by chromatographic methods using TRU Resin (Hellstrom, 2003). In addition to the regular samples, the
internal standards and blank samples were processed by the same procedure. U and Th isotopic compositions of all the samples and standards were
measured at the Institute of Geology of the Czech Academy of Sciences public research institution (Prague, Czech Republic) by a double-focusing sector-field inductively coupled plasma (ICP) mass analyser (Element 2,
Thermo Finnigan MAT). The spectrometer settings were at a low mass resolution (<inline-formula><mml:math id="M51" display="inline"><mml:mrow><mml:mi>m</mml:mi><mml:mo>/</mml:mo><mml:mi mathvariant="normal">Δ</mml:mi><mml:mi>m</mml:mi></mml:mrow></mml:math></inline-formula> <inline-formula><mml:math id="M52" display="inline"><mml:mo>≥</mml:mo></mml:math></inline-formula> 300).</p>

<?xmltex \floatpos{t}?><table-wrap id="Ch1.T1" specific-use="star"><?xmltex \currentcnt{1}?><label>Table 1</label><caption><p id="d1e791">U-series results. AR stands for activity ratio.</p></caption><oasis:table frame="topbot"><oasis:tgroup cols="8">
     <oasis:colspec colnum="1" colname="col1" align="left"/>
     <oasis:colspec colnum="2" colname="col2" align="right"/>
     <oasis:colspec colnum="3" colname="col3" align="right"/>
     <oasis:colspec colnum="4" colname="col4" align="right"/>
     <oasis:colspec colnum="5" colname="col5" align="right"/>
     <oasis:colspec colnum="6" colname="col6" align="right"/>
     <oasis:colspec colnum="7" colname="col7" align="right"/>
     <oasis:colspec colnum="8" colname="col8" align="right"/>
     <oasis:thead>
       <oasis:row>
         <oasis:entry colname="col1"><inline-formula><mml:math id="M55" display="inline"><mml:mrow><mml:msup><mml:mi>H</mml:mi><mml:mi mathvariant="normal">a</mml:mi></mml:msup></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col2">U</oasis:entry>
         <oasis:entry colname="col3"><inline-formula><mml:math id="M56" display="inline"><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">234</mml:mn></mml:msup><mml:mi mathvariant="normal">U</mml:mi></mml:mrow></mml:math></inline-formula>/<inline-formula><mml:math id="M57" display="inline"><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">238</mml:mn></mml:msup><mml:mi mathvariant="normal">U</mml:mi></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col4"><inline-formula><mml:math id="M58" display="inline"><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">230</mml:mn></mml:msup><mml:mi mathvariant="normal">Th</mml:mi></mml:mrow></mml:math></inline-formula>/<inline-formula><mml:math id="M59" display="inline"><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">234</mml:mn></mml:msup><mml:mi mathvariant="normal">U</mml:mi></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col5"><inline-formula><mml:math id="M60" display="inline"><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">230</mml:mn></mml:msup><mml:mi mathvariant="normal">Th</mml:mi></mml:mrow></mml:math></inline-formula>/<inline-formula><mml:math id="M61" display="inline"><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">232</mml:mn></mml:msup><mml:mi mathvariant="normal">Th</mml:mi></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col6">Age</oasis:entry>
         <oasis:entry colname="col7">Corrected age<inline-formula><mml:math id="M62" display="inline"><mml:msup><mml:mi/><mml:mi mathvariant="normal">b</mml:mi></mml:msup></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col8">Initial <inline-formula><mml:math id="M63" display="inline"><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">234</mml:mn></mml:msup><mml:mi mathvariant="normal">U</mml:mi></mml:mrow></mml:math></inline-formula>/<inline-formula><mml:math id="M64" display="inline"><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">238</mml:mn></mml:msup><mml:mi mathvariant="normal">U</mml:mi></mml:mrow></mml:math></inline-formula></oasis:entry>
       </oasis:row>
       <oasis:row rowsep="1">
         <oasis:entry colname="col1">(mm)</oasis:entry>
         <oasis:entry colname="col2">(ppm)</oasis:entry>
         <oasis:entry colname="col3">AR</oasis:entry>
         <oasis:entry colname="col4">AR</oasis:entry>
         <oasis:entry colname="col5">AR</oasis:entry>
         <oasis:entry colname="col6">(ka)</oasis:entry>
         <oasis:entry colname="col7">(ka)</oasis:entry>
         <oasis:entry colname="col8">AR</oasis:entry>
       </oasis:row>
     </oasis:thead>
     <oasis:tbody>
       <oasis:row>
         <oasis:entry colname="col1">3 <inline-formula><mml:math id="M65" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.5</oasis:entry>
         <oasis:entry colname="col2">0.193 <inline-formula><mml:math id="M66" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.001</oasis:entry>
         <oasis:entry colname="col3">1.827 <inline-formula><mml:math id="M67" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.010</oasis:entry>
         <oasis:entry colname="col4">0.785 <inline-formula><mml:math id="M68" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.009</oasis:entry>
         <oasis:entry colname="col5">232 <inline-formula><mml:math id="M69" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 3</oasis:entry>
         <oasis:entry colname="col6">142 <inline-formula><mml:math id="M70" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 3</oasis:entry>
         <oasis:entry colname="col7">140 <inline-formula><mml:math id="M71" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 4</oasis:entry>
         <oasis:entry colname="col8">2.223 <inline-formula><mml:math id="M72" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.065</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">7.5 <inline-formula><mml:math id="M73" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.5</oasis:entry>
         <oasis:entry colname="col2">0.271 <inline-formula><mml:math id="M74" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.002</oasis:entry>
         <oasis:entry colname="col3">2.299 <inline-formula><mml:math id="M75" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.015</oasis:entry>
         <oasis:entry colname="col4">0.788 <inline-formula><mml:math id="M76" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.010</oasis:entry>
         <oasis:entry colname="col5">787 <inline-formula><mml:math id="M77" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 9</oasis:entry>
         <oasis:entry colname="col6">139 <inline-formula><mml:math id="M78" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 3</oasis:entry>
         <oasis:entry colname="col7">137 <inline-formula><mml:math id="M79" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 3</oasis:entry>
         <oasis:entry colname="col8">2.905 <inline-formula><mml:math id="M80" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.066</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">16 <inline-formula><mml:math id="M81" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.5</oasis:entry>
         <oasis:entry colname="col2">0.241 <inline-formula><mml:math id="M82" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.001</oasis:entry>
         <oasis:entry colname="col3">2.750 <inline-formula><mml:math id="M83" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.002</oasis:entry>
         <oasis:entry colname="col4">0.777 <inline-formula><mml:math id="M84" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.006</oasis:entry>
         <oasis:entry colname="col5">453 <inline-formula><mml:math id="M85" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 4</oasis:entry>
         <oasis:entry colname="col6">132 <inline-formula><mml:math id="M86" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 2</oasis:entry>
         <oasis:entry colname="col7">132 <inline-formula><mml:math id="M87" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 2</oasis:entry>
         <oasis:entry colname="col8">3.531 <inline-formula><mml:math id="M88" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.048</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">33 <inline-formula><mml:math id="M89" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.5</oasis:entry>
         <oasis:entry colname="col2">0.284 <inline-formula><mml:math id="M90" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.002</oasis:entry>
         <oasis:entry colname="col3">1.824 <inline-formula><mml:math id="M91" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.011</oasis:entry>
         <oasis:entry colname="col4">0.706 <inline-formula><mml:math id="M92" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.010</oasis:entry>
         <oasis:entry colname="col5">433 <inline-formula><mml:math id="M93" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 6</oasis:entry>
         <oasis:entry colname="col6">118 <inline-formula><mml:math id="M94" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 3</oasis:entry>
         <oasis:entry colname="col7">118 <inline-formula><mml:math id="M95" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 3</oasis:entry>
         <oasis:entry colname="col8">2.146 <inline-formula><mml:math id="M96" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.049</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">43 <inline-formula><mml:math id="M97" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.5</oasis:entry>
         <oasis:entry colname="col2">0.225 <inline-formula><mml:math id="M98" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.002</oasis:entry>
         <oasis:entry colname="col3">2.120 <inline-formula><mml:math id="M99" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.009</oasis:entry>
         <oasis:entry colname="col4">0.692 <inline-formula><mml:math id="M100" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.007</oasis:entry>
         <oasis:entry colname="col5">68 <inline-formula><mml:math id="M101" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 1</oasis:entry>
         <oasis:entry colname="col6">113 <inline-formula><mml:math id="M102" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 2</oasis:entry>
         <oasis:entry colname="col7">111 <inline-formula><mml:math id="M103" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 3</oasis:entry>
         <oasis:entry colname="col8">2.527 <inline-formula><mml:math id="M104" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.062</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">81 <inline-formula><mml:math id="M105" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.5</oasis:entry>
         <oasis:entry colname="col2">0.245 <inline-formula><mml:math id="M106" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.002</oasis:entry>
         <oasis:entry colname="col3">2.000 <inline-formula><mml:math id="M107" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.010</oasis:entry>
         <oasis:entry colname="col4">0.678 <inline-formula><mml:math id="M108" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.010</oasis:entry>
         <oasis:entry colname="col5">429 <inline-formula><mml:math id="M109" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 6</oasis:entry>
         <oasis:entry colname="col6">110 <inline-formula><mml:math id="M110" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 3</oasis:entry>
         <oasis:entry colname="col7">109 <inline-formula><mml:math id="M111" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 3</oasis:entry>
         <oasis:entry colname="col8">2.356 <inline-formula><mml:math id="M112" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.057</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">107 <inline-formula><mml:math id="M113" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.5</oasis:entry>
         <oasis:entry colname="col2">0.295 <inline-formula><mml:math id="M114" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.002</oasis:entry>
         <oasis:entry colname="col3">1.996 <inline-formula><mml:math id="M115" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.011</oasis:entry>
         <oasis:entry colname="col4">0.615 <inline-formula><mml:math id="M116" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.009</oasis:entry>
         <oasis:entry colname="col5">632 <inline-formula><mml:math id="M117" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 9</oasis:entry>
         <oasis:entry colname="col6">95 <inline-formula><mml:math id="M118" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 2</oasis:entry>
         <oasis:entry colname="col7">94 <inline-formula><mml:math id="M119" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 2</oasis:entry>
         <oasis:entry colname="col8">2.295 <inline-formula><mml:math id="M120" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.050</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">128 <inline-formula><mml:math id="M121" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.5</oasis:entry>
         <oasis:entry colname="col2">0.216 <inline-formula><mml:math id="M122" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.002</oasis:entry>
         <oasis:entry colname="col3">1.755 <inline-formula><mml:math id="M123" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.007</oasis:entry>
         <oasis:entry colname="col4">0.606 <inline-formula><mml:math id="M124" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.008</oasis:entry>
         <oasis:entry colname="col5">287 <inline-formula><mml:math id="M125" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 4</oasis:entry>
         <oasis:entry colname="col6">94 <inline-formula><mml:math id="M126" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 2</oasis:entry>
         <oasis:entry colname="col7">92 <inline-formula><mml:math id="M127" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 3</oasis:entry>
         <oasis:entry colname="col8">1.976 <inline-formula><mml:math id="M128" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.056</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">140 <inline-formula><mml:math id="M129" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.5</oasis:entry>
         <oasis:entry colname="col2">0.235 <inline-formula><mml:math id="M130" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.001</oasis:entry>
         <oasis:entry colname="col3">1.742 <inline-formula><mml:math id="M131" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.006</oasis:entry>
         <oasis:entry colname="col4">0.593 <inline-formula><mml:math id="M132" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.007</oasis:entry>
         <oasis:entry colname="col5">90 <inline-formula><mml:math id="M133" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 1</oasis:entry>
         <oasis:entry colname="col6">90 <inline-formula><mml:math id="M134" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 2</oasis:entry>
         <oasis:entry colname="col7">89 <inline-formula><mml:math id="M135" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 2</oasis:entry>
         <oasis:entry colname="col8">1.952 <inline-formula><mml:math id="M136" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.044</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">145 <inline-formula><mml:math id="M137" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.5</oasis:entry>
         <oasis:entry colname="col2">0.296 <inline-formula><mml:math id="M138" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.003</oasis:entry>
         <oasis:entry colname="col3">1.954 <inline-formula><mml:math id="M139" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.013</oasis:entry>
         <oasis:entry colname="col4">0.581 <inline-formula><mml:math id="M140" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.018</oasis:entry>
         <oasis:entry colname="col5">2396 <inline-formula><mml:math id="M141" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 73</oasis:entry>
         <oasis:entry colname="col6">87 <inline-formula><mml:math id="M142" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 1</oasis:entry>
         <oasis:entry colname="col7">87 <inline-formula><mml:math id="M143" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 1</oasis:entry>
         <oasis:entry colname="col8">2.217 <inline-formula><mml:math id="M144" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.023</oasis:entry>
       </oasis:row>
     </oasis:tbody>
   </oasis:tgroup></oasis:table><table-wrap-foot><p id="d1e794"><inline-formula><mml:math id="M53" display="inline"><mml:msup><mml:mi/><mml:mi mathvariant="normal">a</mml:mi></mml:msup></mml:math></inline-formula> Distance from the base. <inline-formula><mml:math id="M54" display="inline"><mml:msup><mml:mi/><mml:mi mathvariant="normal">b</mml:mi></mml:msup></mml:math></inline-formula> Corrected ages using the modified algorithm proposed by Hellstrom (2006).</p></table-wrap-foot></table-wrap>

      <p id="d1e1845">The obtained measurements were corrected for background counts and chemical blanks. The final results were reported as the activity ratios
(Table 1). The final U-series ages were calculated using the latest decay constants (in <inline-formula><mml:math id="M145" display="inline"><mml:mrow class="unit"><mml:msup><mml:mi mathvariant="normal">yr</mml:mi><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:mrow></mml:math></inline-formula>):
<inline-formula><mml:math id="M146" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">λ</mml:mi><mml:mn mathvariant="normal">238</mml:mn></mml:msub></mml:mrow></mml:math></inline-formula> <inline-formula><mml:math id="M147" display="inline"><mml:mo>=</mml:mo></mml:math></inline-formula> (1.55125 <inline-formula><mml:math id="M148" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.0017) <inline-formula><mml:math id="M149" display="inline"><mml:mo>×</mml:mo></mml:math></inline-formula> 10<inline-formula><mml:math id="M150" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">10</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> (Jaffey et al., 1971),
<inline-formula><mml:math id="M151" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">λ</mml:mi><mml:mn mathvariant="normal">234</mml:mn></mml:msub></mml:mrow></mml:math></inline-formula> <inline-formula><mml:math id="M152" display="inline"><mml:mo>=</mml:mo></mml:math></inline-formula> (2.826 <inline-formula><mml:math id="M153" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.0056) <inline-formula><mml:math id="M154" display="inline"><mml:mo>×</mml:mo></mml:math></inline-formula> 10<inline-formula><mml:math id="M155" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">6</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> (Cheng et al., 2013),
<inline-formula><mml:math id="M156" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">λ</mml:mi><mml:mn mathvariant="normal">232</mml:mn></mml:msub></mml:mrow></mml:math></inline-formula> <inline-formula><mml:math id="M157" display="inline"><mml:mo>=</mml:mo></mml:math></inline-formula> (4.95 <inline-formula><mml:math id="M158" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.035) <inline-formula><mml:math id="M159" display="inline"><mml:mo>×</mml:mo></mml:math></inline-formula> 10<inline-formula><mml:math id="M160" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">11</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> (Holden, 1990) and <inline-formula><mml:math id="M161" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">λ</mml:mi><mml:mn mathvariant="normal">230</mml:mn></mml:msub></mml:mrow></mml:math></inline-formula> <inline-formula><mml:math id="M162" display="inline"><mml:mo>=</mml:mo></mml:math></inline-formula> (9.1577 <inline-formula><mml:math id="M163" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.028) <inline-formula><mml:math id="M164" display="inline"><mml:mo>×</mml:mo></mml:math></inline-formula> 10<inline-formula><mml:math id="M165" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">6</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula>
(Cheng et al., 2013). The reported age errors were estimated using error propagation rules. All measurement errors were considered except for the
decay constant.</p>
      <p id="d1e2042">A modified version of the Hellstrom algorithm was applied to correct the obtained ages (Hellstrom, 2006), with the assumption of initial contamination
by <inline-formula><mml:math id="M166" display="inline"><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">230</mml:mn></mml:msup><mml:mi mathvariant="normal">Th</mml:mi></mml:mrow></mml:math></inline-formula>, <inline-formula><mml:math id="M167" display="inline"><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">234</mml:mn></mml:msup><mml:mi mathvariant="normal">U</mml:mi></mml:mrow></mml:math></inline-formula> and <inline-formula><mml:math id="M168" display="inline"><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">238</mml:mn></mml:msup><mml:mi mathvariant="normal">U</mml:mi></mml:mrow></mml:math></inline-formula> isotopes. The algorithm searches for the lowest values of initial contamination by
<inline-formula><mml:math id="M169" display="inline"><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">230</mml:mn></mml:msup><mml:mi mathvariant="normal">Th</mml:mi></mml:mrow></mml:math></inline-formula>, <inline-formula><mml:math id="M170" display="inline"><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">234</mml:mn></mml:msup><mml:mi mathvariant="normal">U</mml:mi></mml:mrow></mml:math></inline-formula> and <inline-formula><mml:math id="M171" display="inline"><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">238</mml:mn></mml:msup><mml:mi mathvariant="normal">U</mml:mi></mml:mrow></mml:math></inline-formula> isotopes from detrital sources, which were able to correct series of ages in stratigraphic order
(Błaszczyk et al., 2021). The age–depth<?pagebreak page1054?> model was calculated using the MOD-AGE algorithm (Hercman and Pawlak, 2012).</p>
</sec>
<sec id="Ch1.S3.SS3">
  <label>3.3</label><title>Stable isotopes</title>
      <p id="d1e2126">The samples for stable isotopic composition measurement were drilled by a Micro Mill with a 0.1 <inline-formula><mml:math id="M172" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">mm</mml:mi></mml:mrow></mml:math></inline-formula> drill bit diameter. The final number of
obtained samples was 290. In the first stage, the JS9 speleothem was sampled along its growth axis at a resolution of
one sample per millimetre. The lower part of the stalagmite (0 to 40 <inline-formula><mml:math id="M173" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">mm</mml:mi></mml:mrow></mml:math></inline-formula>) was additionally sampled at a resolution of
one sample every 0.3 mm to minimise the difference in resolution between the lower and upper parts of the
record caused by differences in growth rate. The isotopic composition of O and C was measured by a Thermo Kiel IV carbonate device connected to a
Finnigan Delta<sup>plus</sup> isotope-ratio mass spectrometer (IRMS) in dual-inlet mode. The results were normalised to three international standards, NBS 19, NBS 18 and IAEA CO 8,
and were reported relative to the Vienna Pee Dee Belemnite (VPDB) international standard. The analytical precision (1<inline-formula><mml:math id="M174" display="inline"><mml:mi mathvariant="italic">σ</mml:mi></mml:math></inline-formula>) was better than 0.03 ‰ and
0.08 ‰ for <inline-formula><mml:math id="M175" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> and <inline-formula><mml:math id="M176" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> respectively. The reproducibility was checked by measurement of two internal
standards after every 12 samples (<inline-formula><mml:math id="M177" display="inline"><mml:mo lspace="0mm">±</mml:mo></mml:math></inline-formula> 0.03 ‰ for <inline-formula><mml:math id="M178" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula>; <inline-formula><mml:math id="M179" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 0.08 ‰ for <inline-formula><mml:math id="M180" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula>). The analyses
were performed in the Stable Isotope Laboratory (Institute of Geological Sciences, Polish Academy of Sciences) in Warsaw.</p>
</sec>
<sec id="Ch1.S3.SS4">
  <label>3.4</label><title>Trace elements</title>
      <p id="d1e2238">The trace element content was analysed from thin sections by an analyte excite excimer laser ablation (LA) system with a wavelength of 193 <inline-formula><mml:math id="M181" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">nm</mml:mi></mml:mrow></mml:math></inline-formula>
connected to an Element 2 ICP mass spectrometer (ICP-MS; Thermo Finnigan); a fluence of 2.44 <inline-formula><mml:math id="M182" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">J</mml:mi><mml:mspace width="0.125em" linebreak="nobreak"/><mml:msup><mml:mi mathvariant="normal">cm</mml:mi><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">2</mml:mn></mml:mrow></mml:msup></mml:mrow></mml:math></inline-formula> was obtained at
50 % laser power with 10 Hz pulses. The width of each line was 50 <inline-formula><mml:math id="M183" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">µ</mml:mi><mml:mi mathvariant="normal">m</mml:mi></mml:mrow></mml:math></inline-formula>, and the laser speed at each scan was
5 <inline-formula><mml:math id="M184" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">µ</mml:mi><mml:mi mathvariant="normal">m</mml:mi><mml:mspace width="0.125em" linebreak="nobreak"/><mml:msup><mml:mi mathvariant="normal">s</mml:mi><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:mrow></mml:math></inline-formula>. Additional details of the LA-ICP-MS analytical procedure followed the procedure described by Eggins
et al. (1997). Measurements of near-surface trace element contents, namely, Mg, Sr, Na, Ba, P Si, Fe and Mn, were performed at medium resolution. The
obtained raw data were normalised to Ca. Finally, the data were smoothed by the adjected averaging method using 10 nearby data points.</p>
</sec>
</sec>
<sec id="Ch1.S4">
  <label>4</label><title>Results</title>
<sec id="Ch1.S4.SS1">
  <label>4.1</label><title>Petrography</title>
      <p id="d1e2311">The results of petrographic studies are presented in Fig. 2. The JS9 sample is a 155 <inline-formula><mml:math id="M185" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">mm</mml:mi></mml:mrow></mml:math></inline-formula> long columnar stalagmite with a diameter of
80 <inline-formula><mml:math id="M186" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">mm</mml:mi></mml:mrow></mml:math></inline-formula>. Macroscopically, the JS9 stalagmite is composed of laminated calcite (Fig. 2a). The colour of the laminae changes from light cream to
dark brown (Fig. 2a). Between 75 and 85 <inline-formula><mml:math id="M187" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">mm</mml:mi></mml:mrow></mml:math></inline-formula>, the stalagmite has a grey colour. The light cream laminae between 40 and 75 <inline-formula><mml:math id="M188" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">mm</mml:mi></mml:mrow></mml:math></inline-formula> have a
zone of macroscopically visible porosity in the axial part of the stalagmite. Microscopic analysis of the appearance of calcite crystals and the
identification of textural features show that most of the observed stalagmite consist of columnar polycrystals with length-to-width ratios that are
usually <inline-formula><mml:math id="M189" display="inline"><mml:mo>&gt;</mml:mo></mml:math></inline-formula> <inline-formula><mml:math id="M190" display="inline"><mml:mrow><mml:mn mathvariant="normal">10</mml:mn><mml:mo>:</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:math></inline-formula>. “Fibre-like” calcite individuals compose each polycrystal. The overall appearance of this layer resembles spherulite, which is
composed of bundles of elongated crystals bent outward (Fig. 2b, c). The polycrystals show brush extinction that converges away from the substrate
when the rotating table is turned clockwise. The characteristics mentioned above are similar to those described by Frisia (2015); these
characteristics indicate that the columnar radiaxial fibrous (Crf) texture is a dominant fabric in the JS9 stalagmite (Fig. 2b). Parts of stalagmites containing Crf fabric are usually separated by thin layers of small calcite crystals and detrital material – those layers are dark in cross-polarised light (Fig. 2c). The appearance of these thin layers indicates the micrite (M) fabric described by Frisia (2015). Micrite fabric is most abundant in the
middle part and in the youngest layers of the JS9 stalagmite (Fig. 2c).</p>

      <?xmltex \floatpos{t}?><fig id="Ch1.F2"><?xmltex \currentcnt{2}?><?xmltex \def\figurename{Figure}?><label>Figure 2</label><caption><p id="d1e2368">The JS9 sample lithology: <bold>(a)</bold> a photo of the JS9 stalagmite, <bold>(b)</bold> a microfabric log scaled by distance from the base of the stalagmite and <bold>(c)</bold> exemplary photos of microfabrics. M denotes micrite fabric, and Crf denotes columnar radiaxial fibrous microfabric.</p></caption>
          <?xmltex \igopts{width=241.848425pt}?><graphic xlink:href="https://cp.copernicus.org/articles/17/1051/2021/cp-17-1051-2021-f02.png"/>

        </fig>

<?xmltex \hack{\newpage}?>
</sec>
<?pagebreak page1055?><sec id="Ch1.S4.SS2">
  <label>4.2</label><title>U-series dating and age–depth model construction</title>
      <p id="d1e2396">The results of 10 U-series dates are presented in Table 1. The reported errors are 2<inline-formula><mml:math id="M191" display="inline"><mml:mi mathvariant="italic">σ</mml:mi></mml:math></inline-formula> and vary from 0.8 % to 2.8 %. The analysed
samples do not show any visible detrital contamination at the dissolution stage. However, four of the measured samples have a
<inline-formula><mml:math id="M192" display="inline"><mml:mrow><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">230</mml:mn></mml:msup><mml:mi mathvariant="normal">Th</mml:mi></mml:mrow><mml:mo>/</mml:mo><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">232</mml:mn></mml:msup><mml:mi mathvariant="normal">Th</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> ratio lower than 300. In the case of measurement by mass spectrometry, these samples should be considered
contaminated by detrital admixtures (Hellstrom, 2006). Therefore, the entire profile was corrected using a modified procedure proposed by Hellstrom
(2006). The result of correction shows that the corrected ages are within the error range of the uncorrected ages (Table 1).</p>
      <p id="d1e2428">Based on the results of U-series dating, an age–depth model was constructed for the JS9 stalagmite (Fig. 3). According to the obtained age–depth model,
deposition of the JS9 stalagmite started at 142 <inline-formula><mml:math id="M193" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 4 <inline-formula><mml:math id="M194" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> and ended at 84 <inline-formula><mml:math id="M195" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 3 <inline-formula><mml:math id="M196" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>. The growth rate of the JS9 stalagmite was
not uniform. From 142 to 112 <inline-formula><mml:math id="M197" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>, its growth rate was relatively slow at 1.4 <inline-formula><mml:math id="M198" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">mm</mml:mi><mml:mspace width="0.125em" linebreak="nobreak"/><mml:msup><mml:mi mathvariant="normal">kyr</mml:mi><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:mrow></mml:math></inline-formula>; after 112 <inline-formula><mml:math id="M199" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>, it rapidly grew at a
rate of 11.5 <inline-formula><mml:math id="M200" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">mm</mml:mi><mml:mspace linebreak="nobreak" width="0.125em"/><mml:msup><mml:mi mathvariant="normal">kyr</mml:mi><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:mrow></mml:math></inline-formula>, which ended at 109 <inline-formula><mml:math id="M201" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>, and then the growth rate slowed to 1.9 <inline-formula><mml:math id="M202" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">mm</mml:mi><mml:mspace width="0.125em" linebreak="nobreak"/><mml:msup><mml:mi mathvariant="normal">kyr</mml:mi><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:mrow></mml:math></inline-formula>. The last intensive change
in the growth rate occurred after 95 <inline-formula><mml:math id="M203" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> when it increased to 4.2 <inline-formula><mml:math id="M204" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">mm</mml:mi><mml:mspace width="0.125em" linebreak="nobreak"/><mml:msup><mml:mi mathvariant="normal">kyr</mml:mi><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:mrow></mml:math></inline-formula>.</p>

      <?xmltex \floatpos{t}?><fig id="Ch1.F3"><?xmltex \currentcnt{3}?><?xmltex \def\figurename{Figure}?><label>Figure 3</label><caption><p id="d1e2565">Age–depth model for the JS9 stalagmite. 1 denotes the age–depth model median, 2 denotes the 2<inline-formula><mml:math id="M205" display="inline"><mml:mi mathvariant="italic">σ</mml:mi></mml:math></inline-formula> confidence band and 3 denotes the U-series ages with 2<inline-formula><mml:math id="M206" display="inline"><mml:mi mathvariant="italic">σ</mml:mi></mml:math></inline-formula> uncertainties.</p></caption>
          <?xmltex \igopts{width=213.395669pt}?><graphic xlink:href="https://cp.copernicus.org/articles/17/1051/2021/cp-17-1051-2021-f03.png"/>

        </fig>

<?xmltex \hack{\newpage}?>
</sec>
<sec id="Ch1.S4.SS3">
  <label>4.3</label><title>Stable isotopes</title>
      <p id="d1e2598">The obtained isotopic records (Fig. 4a, b) cover the interval from the late MIS 6 to MIS 5a. The mean value of the <inline-formula><mml:math id="M207" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> record is
<inline-formula><mml:math id="M208" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>7.05 ‰ (Fig. 4a), and its value varies in a range from <inline-formula><mml:math id="M209" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>9 ‰ to <inline-formula><mml:math id="M210" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>5.7 ‰. The <inline-formula><mml:math id="M211" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> signal expresses
short time changes, and the average value of its amplitude is ca. 0.8 ‰ (Fig. 4a). At the end of MIS 6, an increase in <inline-formula><mml:math id="M212" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula>
values is interrupted by a 1.2 ‰ instant decline. The next important change occurs at the boundary between MIS 5d and MIS 5c – an episode of
elevated <inline-formula><mml:math id="M213" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> values greater than <inline-formula><mml:math id="M214" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>6 ‰. Finally, a short episode occurs at the boundary between MIS 5c and MIS 5b, where
<inline-formula><mml:math id="M215" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> values decrease to <inline-formula><mml:math id="M216" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>9 ‰ (Fig. 4a). The <inline-formula><mml:math id="M217" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> record expresses changes in its values from
<inline-formula><mml:math id="M218" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>1 ‰ to <inline-formula><mml:math id="M219" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>9.8 ‰ (Fig. 4b). The average amplitude of <inline-formula><mml:math id="M220" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> values for changes over a short period is close to
1 ‰. In contrast to the <inline-formula><mml:math id="M221" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> record, the <inline-formula><mml:math id="M222" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> curve is dominated by episodes of lower and higher
values. They are divided by large-scale shifts (Fig. 4b). From 143 to 139 <inline-formula><mml:math id="M223" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>, <inline-formula><mml:math id="M224" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> values rise to <inline-formula><mml:math id="M225" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>1 ‰. From 139
to 130 <inline-formula><mml:math id="M226" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> (an interval of 9 <inline-formula><mml:math id="M227" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>), <inline-formula><mml:math id="M228" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> values decrease from <inline-formula><mml:math id="M229" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>2 ‰ to <inline-formula><mml:math id="M230" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>7 ‰ (5 ‰), and the values are
low ca. <inline-formula><mml:math id="M231" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>8 ‰ until 110 <inline-formula><mml:math id="M232" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>. From 110 to 107 <inline-formula><mml:math id="M233" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>, <inline-formula><mml:math id="M234" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> values increase from <inline-formula><mml:math id="M235" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>9.3 ‰ to <inline-formula><mml:math id="M236" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>2.6 ‰
(6.7 ‰) and decrease from <inline-formula><mml:math id="M237" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>1 ‰ to <inline-formula><mml:math id="M238" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>8.5 ‰ (7 ‰) at 101 <inline-formula><mml:math id="M239" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>. From 100 to 85 <inline-formula><mml:math id="M240" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>, <inline-formula><mml:math id="M241" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> values
oscillate at approximately <inline-formula><mml:math id="M242" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>8.2 ‰. After 85 <inline-formula><mml:math id="M243" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>, <inline-formula><mml:math id="M244" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> values increase to <inline-formula><mml:math id="M245" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>5.6 ‰ (Fig. 4b).</p>

      <?xmltex \floatpos{p}?><fig id="Ch1.F4" specific-use="star"><?xmltex \currentcnt{4}?><?xmltex \def\figurename{Figure}?><label>Figure 4</label><caption><p id="d1e3002">Results of multiproxy analyses of the JS9 stalagmite: <bold>(a)</bold> <inline-formula><mml:math id="M246" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> composition with the mean value for the Holocene from DCS shown as a green line, <bold>(b)</bold> <inline-formula><mml:math id="M247" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> composition, <bold>(c)</bold> Mg content, <bold>(d)</bold> Sr content, <bold>(e)</bold> Ba content, <bold>(f)</bold> Na content, <bold>(g)</bold> P content, <bold>(h)</bold> Fe content, <bold>(i)</bold> Mn content, <bold>(j)</bold> Si content and <bold>(k)</bold> the microfabrics log. The data are available at <uri>https://doi.org/10.6084/m9.figshare.13116506.v1</uri>.</p></caption>
          <?xmltex \igopts{width=224.776772pt}?><graphic xlink:href="https://cp.copernicus.org/articles/17/1051/2021/cp-17-1051-2021-f04.png"/>

        </fig>

</sec>
<sec id="Ch1.S4.SS4">
  <label>4.4</label><title>Trace elements</title>
      <?pagebreak page1057?><p id="d1e3087">The trace element content results are presented in Fig. 4c–j. Mg, Sr and Ba contents do not show clear correlations or anticorrelations
(Fig. 4c–e). However, a few single Mg extremes are clearly in phase or out of phase with the Sr content. For example, at 138 <inline-formula><mml:math id="M248" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>, they are in
phase, and at 122.5 <inline-formula><mml:math id="M249" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>, they are out of phase (Fig. 4c, d). Generally, Mg and Ba records are similar from 138 to 101 <inline-formula><mml:math id="M250" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>, whereas Sr
and Ba contents show more similarities before 138 <inline-formula><mml:math id="M251" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> and after 101 <inline-formula><mml:math id="M252" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>. Mg and Sr contents have minimums at 101 <inline-formula><mml:math id="M253" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>, whereas the
minimum Ba content occurs at 93 <inline-formula><mml:math id="M254" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> and is mimicked by a decreasing Sr content during the same period.</p>
      <p id="d1e3147">Na, P, Fe and Mn contents show a similar pattern (Fig. 4f–i), and they have three intervals of increased values: before 138 <inline-formula><mml:math id="M255" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>, from 106 to
98 <inline-formula><mml:math id="M256" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> and after 92.5 <inline-formula><mml:math id="M257" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>. From 98 to 92 <inline-formula><mml:math id="M258" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>, Na, P, Fe and Mn contents are lower. This trend is also similar for Ba and
Sr contents, and only the Mg content has a different pattern in this interval. The Fe content has the largest number of peaks (Fig. 4h), several of
which are also shown by Na, P and Mn contents (Fig. 4f, g, and i respectively). In comparison to those four records, the P content has the lowest peak amplitude
(Fig. 4g).</p>
      <p id="d1e3182">The Si content shows a few different patterns. The most visible pattern is a short maximum at 102 <inline-formula><mml:math id="M259" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>, which transitions into a minimum almost
immediately at 101 <inline-formula><mml:math id="M260" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>, but the amplitude of changes in the Si content is relatively low. However, from 122 to 102 <inline-formula><mml:math id="M261" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>, it has an
increasing trend, and a similar trend can be observed for Na and P contents (Fig. 4f, g, j). Similar to the Fe, Mn, P, Na and Ba contents, the
Si content has higher values before 138 <inline-formula><mml:math id="M262" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> and after 98 <inline-formula><mml:math id="M263" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>.</p>
</sec>
</sec>
<sec id="Ch1.S5">
  <label>5</label><title>Discussion</title>
<sec id="Ch1.S5.SS1">
  <label>5.1</label><?xmltex \opttitle{Drivers of $\delta{\protect\chem{{}^{{18}}O}}$ in DCS speleothems}?><title>Drivers of <inline-formula><mml:math id="M264" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> in DCS speleothems</title>
      <p id="d1e3258">The meteorological data collected in Slovakia show that there is a significant dependency between the <inline-formula><mml:math id="M265" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value of atmospheric
precipitation and the mean annual temperature (<inline-formula><mml:math id="M266" display="inline"><mml:mrow><mml:msup><mml:mi>R</mml:mi><mml:mn mathvariant="normal">2</mml:mn></mml:msup></mml:mrow></mml:math></inline-formula> <inline-formula><mml:math id="M267" display="inline"><mml:mo>=</mml:mo></mml:math></inline-formula> 0.73; Holko et al., 2012). The influence of the annual amount of precipitation on the
<inline-formula><mml:math id="M268" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value is less obvious (<inline-formula><mml:math id="M269" display="inline"><mml:mrow><mml:msup><mml:mi>R</mml:mi><mml:mn mathvariant="normal">2</mml:mn></mml:msup></mml:mrow></mml:math></inline-formula> <inline-formula><mml:math id="M270" display="inline"><mml:mo>=</mml:mo></mml:math></inline-formula> 0.48; Holko et al., 2012). In the DCS region, the temperature gradient for the
<inline-formula><mml:math id="M271" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> of precipitation is 0.36 <inline-formula><mml:math id="M272" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">‰</mml:mi><mml:mspace linebreak="nobreak" width="0.125em"/><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup><mml:msup><mml:mi mathvariant="normal">C</mml:mi><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:mrow></mml:math></inline-formula> (Holko et al., 2012). The current
<inline-formula><mml:math id="M273" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> precipitation value changes during the year from <inline-formula><mml:math id="M274" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>16 ‰ in February to <inline-formula><mml:math id="M275" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>6 ‰ in July (Holko et al.,
2012). Currently, nearly 65 % of precipitation occurs during the spring and summer months (April–September). Therefore, the seepage water is
biased by the seasonal effect, and its mean <inline-formula><mml:math id="M276" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value is higher than expected based on the mean annual temperature.</p>
      <p id="d1e3409">Recently, a meta-analysis of cave drip water and precipitation monitoring records showed that the isotopic composition of dripping water is not affected by evaporation in climates with mean annual temperatures lower than
<inline-formula><mml:math id="M277" display="inline"><mml:mo>+</mml:mo></mml:math></inline-formula>15 <inline-formula><mml:math id="M278" display="inline"><mml:mrow class="unit"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:math></inline-formula> and aridity indices higher than 0.65 (Baker
et al., 2019). The current climate of the study region fits the above-mentioned mean annual temperature and aridity index conditions. Therefore, current interglacial conditions are more conducive for <inline-formula><mml:math id="M279" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> signals to reflect the
regional temperature conditions and isotopic composition of meteoric water. In contrast, during glacial episodes, when the local climate was more
continental and the aridity index was lower, the studied <inline-formula><mml:math id="M280" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> record could have theoretically been affected by the evaporation
effect. However, due to the low mean annual temperature, the effect should not be high.</p>

      <?xmltex \floatpos{t}?><fig id="Ch1.F5" specific-use="star"><?xmltex \currentcnt{5}?><?xmltex \def\figurename{Figure}?><label>Figure 5</label><caption><p id="d1e3463">The locations of the European and Middle Eastern MIS 5–MIS 6 speleothem sites. The numbers used in the panel legend refer to the following: 1 – speleothems with temperature as a dominant factor influencing the <inline-formula><mml:math id="M281" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value, 2 – speleothems for which the changes in the isotopic composition of rainwater and the amount of precipitation are dominant factors influencing the <inline-formula><mml:math id="M282" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value and 3 – the study site.</p></caption>
          <?xmltex \igopts{width=367.040551pt}?><graphic xlink:href="https://cp.copernicus.org/articles/17/1051/2021/cp-17-1051-2021-f05.png"/>

        </fig>

      <p id="d1e3503">The main factor shaping the <inline-formula><mml:math id="M283" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value of western and central European speleothems during the Holocene and last interglacial period
was the temperature (Moseley et al., 2015; Kern et al., 2019; Comas-Bru et al., 2020; Hercman et al., 2020). For example, stalagmites from Cobre Cave,
which is located on the northern coast of Spain (Fig. 5), reflect changes in the oceanic moisture isotopic composition, which is dependent on
temperature (Fig. 6; Rossi et al., 2014). Similarly, the <inline-formula><mml:math id="M284" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> record from Han-sur-Lesse Cave in Belgium (Fig. 6) is driven by
temperature and by changes in the isotopic composition of the ocean surface (Vansteenberge et al., 2016). The main trend of the <inline-formula><mml:math id="M285" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula>
record from the Hungarian stalagmite collected in Baradla Cave (Fig. 5) reflects temperature changes (Demény, 2017).</p>

      <?xmltex \floatpos{p}?><fig id="Ch1.F6" specific-use="star"><?xmltex \currentcnt{6}?><?xmltex \def\figurename{Figure}?><label>Figure 6</label><caption><p id="d1e3553">Comparison of the JS9 <inline-formula><mml:math id="M286" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> record with other records of MIS 5–MIS 6 age from Europe and the Middle East. The records used for the comparison are as follows: NGRIP (Andersen et al., 2004); Magurska (Pawlak et al., 2020), Baradla (Demény et al., 2017); Orlova Tchuka (Pawlak et al., 2019), Schneckenloch (Mosley et al., 2015), Hölloch (Moseley et al., 2015), NALPS (Boch et al., 2011), Entrische Kirche (Meyer et al., 2008), Bourgeois-Delaunay (Couchoud et al., 2009), Cobre (Rossi et al., 2014), Han-sur-Lesse (Vansteenberge et al., 2016), Antro del Corchia (Drysdale et al., 2005), Soreq (Bar-Matthews et al., 2003), Peqiin (Bar-Matthews et al., 2003) and Kanaan (Nehme et al., 2015). The black and grey lines refer to speleothems where temperature is a dominant factor influencing the <inline-formula><mml:math id="M287" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value, green lines denote speleothems where changes in the isotopic composition of rainwater and the amount of precipitation are dominant factors influencing the <inline-formula><mml:math id="M288" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value, and the red line denotes the study site.</p></caption>
          <?xmltex \igopts{width=194.901378pt}?><graphic xlink:href="https://cp.copernicus.org/articles/17/1051/2021/cp-17-1051-2021-f06.png"/>

        </fig>

      <p id="d1e3607">According to the facts listed above, it can be assumed that, in the long term, the temperature effect on atmospheric precipitation is the main factor
shaping the <inline-formula><mml:math id="M289" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value of the DCS stalagmites.</p>
      <p id="d1e3625">However, in the short term, the <inline-formula><mml:math id="M290" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value can be additionally affected by humidity and aridity effects, as described in the
case of the Bourgeois–Delaunay Cave (Couchoud et al., 2009).</p>
      <p id="d1e3643">The whole temperature effect on the <inline-formula><mml:math id="M291" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value of the DCS speleothems calcite is lower than the presently observed <inline-formula><mml:math id="M292" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula>
temperature gradient of local precipitation (0.36 <inline-formula><mml:math id="M293" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">‰</mml:mi><mml:mspace linebreak="nobreak" width="0.125em"/><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup><mml:msup><mml:mi mathvariant="normal">C</mml:mi><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:mrow></mml:math></inline-formula>; Holko et al., 2012). It is caused by
the opposite temperature effect on calcite crystallisation (<inline-formula><mml:math id="M294" display="inline"><mml:mo lspace="0mm">-</mml:mo></mml:math></inline-formula>0.18 <inline-formula><mml:math id="M295" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">‰</mml:mi><mml:mspace width="0.125em" linebreak="nobreak"/><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup><mml:msup><mml:mi mathvariant="normal">C</mml:mi><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:mrow></mml:math></inline-formula>; Tremaine et al., 2011). The expected net
temperature gradient is ca 0.18 <inline-formula><mml:math id="M296" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">‰</mml:mi><mml:mspace linebreak="nobreak" width="0.125em"/><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup><mml:msup><mml:mi mathvariant="normal">C</mml:mi><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:mrow></mml:math></inline-formula>.</p>
</sec>
<sec id="Ch1.S5.SS2">
  <label>5.2</label><?xmltex \opttitle{Drivers of $\delta{\protect\chem{{}^{{13}}C}}$ and trace elements in DCS speleothems}?><title>Drivers of <inline-formula><mml:math id="M297" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> and trace elements in DCS speleothems</title>
      <p id="d1e3771">The <inline-formula><mml:math id="M298" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value of speleothem calcite depends on the proportion of <inline-formula><mml:math id="M299" display="inline"><mml:mrow class="chem"><mml:msub><mml:mi mathvariant="normal">CO</mml:mi><mml:mn mathvariant="normal">2</mml:mn></mml:msub></mml:mrow></mml:math></inline-formula> from a soil source and from a host rock
source. <inline-formula><mml:math id="M300" display="inline"><mml:mrow class="chem"><mml:msub><mml:mi mathvariant="normal">CO</mml:mi><mml:mn mathvariant="normal">2</mml:mn></mml:msub></mml:mrow></mml:math></inline-formula> from a soil source can reflect the changes in the isotopic composition of atmospheric <inline-formula><mml:math id="M301" display="inline"><mml:mrow class="chem"><mml:msub><mml:mi mathvariant="normal">CO</mml:mi><mml:mn mathvariant="normal">2</mml:mn></mml:msub></mml:mrow></mml:math></inline-formula> and is also enriched
in <inline-formula><mml:math id="M302" display="inline"><mml:msup><mml:mi/><mml:mn mathvariant="normal">12</mml:mn></mml:msup></mml:math></inline-formula>C due to biological activity. Due to that fact, a well-developed soil cover results in a lower <inline-formula><mml:math id="M303" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value. The level of
soil development depends on climatic conditions such as temperature and humidity. Currently, the vegetation cover over the DCS is dominated by mixed
forests, including mountain-type forests and grasslands connected with mountain slope activity (Hercman et al., 2020). During the cold episodes of the
last glacial period, the Slovakian landscape was dominated by boreal trees, tundra dwarf shrubs and grasslands (Feurdean et al., 2014; Jankovska
et al., 2002). The beginning of the Holocene was marked by the development of temperate-type forests (Feurdean et al., 2014). Recently, a set of five
Holocene speleothems from different parts of the DCS was investigated (Hercman et al., 2020). The mean <inline-formula><mml:math id="M304" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value varied from
ca. <inline-formula><mml:math id="M305" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>8 ‰ to ca. <inline-formula><mml:math id="M306" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>2 ‰ and was site-dependent (Hercman et al., 2020). Despite these values, the shape of all Holocene
<inline-formula><mml:math id="M307" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> records from the DCS reflects the changes<?pagebreak page1058?> from boreal and tundra vegetation types to temperate and mountain forest types. This
change suggests that the <inline-formula><mml:math id="M308" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> proxy in the DCS can be interpreted in terms of vegetation changes.</p>
      <p id="d1e3906">Trace elements such as Mg, Sr and Ba are transported in water solution. Their relative abundance depends on the time of water residence and on the
host rock composition (Fairchild and Treble, 2009). Drier conditions result in longer water residence times. The process of dolomite rock dissolution
is slower than the process of limestone rock dissolution. Therefore, during longer water residence times, the contribution of trace elements from
dolomite host rock sources increases. Dolomite normally contains less Sr and Ba than calcite, resulting in higher <inline-formula><mml:math id="M309" display="inline"><mml:mrow class="chem"><mml:mi mathvariant="normal">Mg</mml:mi><mml:mo>/</mml:mo><mml:mi mathvariant="normal">Ca</mml:mi></mml:mrow></mml:math></inline-formula> and lower <inline-formula><mml:math id="M310" display="inline"><mml:mrow class="chem"><mml:mi mathvariant="normal">Sr</mml:mi><mml:mo>/</mml:mo><mml:mi mathvariant="normal">Ca</mml:mi></mml:mrow></mml:math></inline-formula>
and <inline-formula><mml:math id="M311" display="inline"><mml:mrow class="chem"><mml:mi mathvariant="normal">Ba</mml:mi><mml:mo>/</mml:mo><mml:mi mathvariant="normal">Ca</mml:mi></mml:mrow></mml:math></inline-formula> ratios during drier conditions (Roberts et al., 1998; Tremaine and Froelich, 2013; Rossi et al., 2014). The DCS is developed mostly
in Gutenstein limestones and Ramsau dolomites (Gaál, 2016). Therefore, the dissolution of both limestones and dolomites is possible in the DCS. On
the other hand, prior calcite precipitation (PCP) can also occur during dry episodes when the water residence time is longer. However, PCP results
in an increase in all <inline-formula><mml:math id="M312" display="inline"><mml:mrow class="chem"><mml:mi mathvariant="normal">X</mml:mi><mml:mo>/</mml:mo><mml:mi mathvariant="normal">Ca</mml:mi></mml:mrow></mml:math></inline-formula> ratios because <inline-formula><mml:math id="M313" display="inline"><mml:mrow class="chem"><mml:msup><mml:mi mathvariant="normal">Ca</mml:mi><mml:mrow><mml:mn mathvariant="normal">2</mml:mn><mml:mo>+</mml:mo></mml:mrow></mml:msup></mml:mrow></mml:math></inline-formula> cations are preferred during calcite crystallisation (Tremaine and Froelich,
2013). Episodes of synchronous increases in <inline-formula><mml:math id="M314" display="inline"><mml:mrow class="chem"><mml:mi mathvariant="normal">Mg</mml:mi><mml:mo>/</mml:mo><mml:mi mathvariant="normal">Ca</mml:mi></mml:mrow></mml:math></inline-formula> and <inline-formula><mml:math id="M315" display="inline"><mml:mrow class="chem"><mml:mi mathvariant="normal">Sr</mml:mi><mml:mo>/</mml:mo><mml:mi mathvariant="normal">Ca</mml:mi></mml:mrow></mml:math></inline-formula> ratios are not observed in the record in this study.</p>
      <p id="d1e3996">Elements, such as Fe, Mn and Si, may be transported as detrital particles or submicron-size colloids (Fairchild and Treble, 2009). Additionally, all
elements that can be incorporated into the calcite structure can be transported as absorbed ions on the clay mineral structure. During drier periods
under higher aeolian supply conditions, particles can be transported into the cave environment without water transportation (Hu et al., 2005).</p>
</sec>
<sec id="Ch1.S5.SS3">
  <label>5.3</label><title>Temporal evolution of environmental proxies in the JS9 speleothem</title>
      <p id="d1e4007">Recently, a set of five speleothems of Holocene age from the DCS was investigated (Hercman et al., 2020). Holocene <inline-formula><mml:math id="M316" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> records from
DCS speleothems reflect the same pattern (Hercman et al., 2020). Additionally, the mean value for all of these records is similar. This proves that the
<inline-formula><mml:math id="M317" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value from DCS speleothems reflects the final regional climatic conditions. Comparing Holocene <inline-formula><mml:math id="M318" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> values with
the record in this study can be useful for its interpretation. A total of 95 % percent of Holocene <inline-formula><mml:math id="M319" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> values from the DCS are in the
range from <inline-formula><mml:math id="M320" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>7.6 % to <inline-formula><mml:math id="M321" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>6.8 % with a mean value of <inline-formula><mml:math id="M322" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>7.2 ‰ (Fig. 4a). There are five periods in which the <inline-formula><mml:math id="M323" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula>
value of the JS9 stalagmite was slightly different than the <inline-formula><mml:math id="M324" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> mean value of the Holocene: 143–135, 127–123, 116–113, 108–101
and 94–92 <inline-formula><mml:math id="M325" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> (Fig. 4a).</p>
      <?pagebreak page1060?><p id="d1e4130"><?xmltex \hack{\newpage}?>The <inline-formula><mml:math id="M326" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> and <inline-formula><mml:math id="M327" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> values in the JS9 stalagmite change dynamically during the 143–130 <inline-formula><mml:math id="M328" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> period. The short
episode of elevated <inline-formula><mml:math id="M329" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> and <inline-formula><mml:math id="M330" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> values from 143 to 137 <inline-formula><mml:math id="M331" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> may be the result of drier conditions with a higher level
of evaporation at the end of MIS 6 (Gascoyne, 1992; Genty et al., 2006, Couchoud et al., 2009). During the 143–137 <inline-formula><mml:math id="M332" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> time interval, Mg and
Ba contents are elevated, whereas the Sr content is lower (Fig. 4c–e). Trace element contents (Mg, Ba and Sr) support this interpretation. From 143 to
137 <inline-formula><mml:math id="M333" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>, the Fe, Mn and Si contents are elevated. This result may be related to the lack of developed soil cover and increased frost erosion of
the surface above the cave. The other explanation of elevated values of listed proxies is the presence of micrite (Fig. 4k). However, this explanation
also leads to dryer conditions (Frisia, 2015). New data revealing <inline-formula><mml:math id="M334" display="inline"><mml:mrow class="chem"><mml:msub><mml:mi mathvariant="normal">CO</mml:mi><mml:mn mathvariant="normal">2</mml:mn></mml:msub></mml:mrow></mml:math></inline-formula> concentration changes during MIS 6 (Shin et al., 2020) show a local
minimum <inline-formula><mml:math id="M335" display="inline"><mml:mrow class="chem"><mml:msub><mml:mi mathvariant="normal">CO</mml:mi><mml:mn mathvariant="normal">2</mml:mn></mml:msub></mml:mrow></mml:math></inline-formula> concentration between 142 and 138 <inline-formula><mml:math id="M336" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>. Therefore, the episode recorded in the JS9 stalagmite may be global in nature.</p>
      <p id="d1e4257">After 137 <inline-formula><mml:math id="M337" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>, the change in the negative value observed in the <inline-formula><mml:math id="M338" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> record (ca. 5 ‰) is ca. 2 times larger than
that observed in the DCS <inline-formula><mml:math id="M339" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> records at the beginning of the Holocene (ca. 2.5 ‰; Hercman et al., 2020). This may reflect
the more substantial change in the environment from periglacial tundra conditions to temperate forests. According to data from other speleothems, the
long-term tendencies of <inline-formula><mml:math id="M340" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> and <inline-formula><mml:math id="M341" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> are clearly related to improvements in thermal conditions after the MIS 6
glacial maximum and before the MIS 6–MIS 5e transition (Pawlak et al., 2020; Moseley et al., 2015; Meyer et al., 2008; Holzkamper et al., 2004).</p>
      <p id="d1e4328">Termination II (T II) in the JS9 stalagmite is highlighted by rapid decrease of ca. 1.2 ‰ in the <inline-formula><mml:math id="M342" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value (Fig. 4a). This
decrease is in contrast to the <inline-formula><mml:math id="M343" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> signal recorded in speleothems from the northern rim of the European Alps (NALPS; Fig. 6). The
observed positive shift in Alpine <inline-formula><mml:math id="M344" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> records during T II is the result of two processes: the improvement of thermal
conditions and the change from winter-dominated precipitation to summer-dominated precipitation (Moseley et al., 2015; Meyer et al., 2008; Holzkamper
et al., 2004; Fig. 6). Similarly, in the caves on the northern slopes of the Tatra Mountains, T II is visible as a positive change in
<inline-formula><mml:math id="M345" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> (Pawlak et al., 2020). However, in the case of the <inline-formula><mml:math id="M346" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> record from Magurska Cave (Tatra Mountains, Poland), the
change towards positive values is preceded by an instant decrease of ca. 2 ‰ in its value (Fig. 6). The difference between the Low Tatras and caves located on the northern slopes of the Tatra Mountains (ca. 39 <inline-formula><mml:math id="M347" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">km</mml:mi></mml:mrow></mml:math></inline-formula> towards the NE) is that the Tatra Mountains were an
important climatic barrier for moisture at that time. In the case of the JS9 stalagmite, the <inline-formula><mml:math id="M348" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> and trace element contents did not
show any signal that could be equivalent to the rapid 1.2 ‰ negative change in the <inline-formula><mml:math id="M349" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> record at 130 <inline-formula><mml:math id="M350" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>
(Fig. 4). Therefore, the recorded 1.2 ‰ negative shift must be caused by factors that affect only the <inline-formula><mml:math id="M351" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula>
proxy. Additionally, the <inline-formula><mml:math id="M352" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value after T II remains at the lower level until the end of MIS 5e. Its average value for MIS 5e
(<inline-formula><mml:math id="M353" display="inline"><mml:mo lspace="0mm">-</mml:mo></mml:math></inline-formula>7.6 ‰) is ca. 0.4 ‰ lower than the average <inline-formula><mml:math id="M354" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value (<inline-formula><mml:math id="M355" display="inline"><mml:mo lspace="0mm">-</mml:mo></mml:math></inline-formula>7.2 ‰) for Holocene speleothems of the DCS
(Hercman et al., 2020). According to the present temperature gradient in Slovakia, the <inline-formula><mml:math id="M356" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>0.4 ‰ difference could be interpreted as a lower
mean temperature of MIS 5e of ca. 1 <inline-formula><mml:math id="M357" display="inline"><mml:mrow class="unit"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:math></inline-formula> in comparison to the Holocene. However, the probability of this simple interpretation is
low. Other reasons, such as changes from summer-dominated precipitation to winter-dominated precipitation or more humid conditions than at present,
are more probable. Generally, in central Europe, the beginning of MIS 5e is related to changes from continental to more transient climates (Demény
et al., 2017; Moseley et al., 2015). Therefore, the other reason for the observed shift may be the source effect and rapid increase in the depletion
of <inline-formula><mml:math id="M358" display="inline"><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:math></inline-formula> moisture from the Atlantic source. The negative shift in the <inline-formula><mml:math id="M359" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> record observed in JS9 speleothems is similar to
the change observed in Mediterranean records (Antro del Corchia, Soreq, Peqiin; Fig. 6). This type of shift in Mediterranean records can be explained
as a source effect when the change in the <inline-formula><mml:math id="M360" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> composition of speleothems is caused by the change in the <inline-formula><mml:math id="M361" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value
of the Mediterranean Sea surface, which is documented by marine cores, and the change in the proportion of moisture from Atlantic and Mediterranean
sources (Bar-Matthews et al., 2003; Nehme et al., 2015). These are similar to records from Antro del Corchia (CC5 stalagmite; Drysdale et al., 2005),
which has an approximately 2.5 ‰ decrease in its <inline-formula><mml:math id="M362" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> values during T II at ca. 130 <inline-formula><mml:math id="M363" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> (Fig. 6). In the
case of the JS9 stalagmite, the observed 1.2 ‰ negative change is the result of a muted response. This response must have been caused by
local or regional effects, which were stronger than the thermal effect at that time. The possible effect that may have caused the lower
<inline-formula><mml:math id="M364" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value is the circulation effect and changes from sources of precipitation, such as the Adriatic Sea or the Black Sea, to
Atlantic sources and vapour recycling over the European continent (Drysdale et al., 2005). The instant change to negative values may have been caused
by source and continental effects, which overcame the temperature effect at that time.</p>
      <?pagebreak page1061?><p id="d1e4629">The MIS 5e interval in the JS9 stalagmite can be divided into two parts. The first part (127–123 <inline-formula><mml:math id="M365" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>) has lower values of
<inline-formula><mml:math id="M366" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> and <inline-formula><mml:math id="M367" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> (Fig. 4a, b). The Mg content during this period is lower than its average value, with a local
minimum at 124 <inline-formula><mml:math id="M368" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> (Fig. 4c). Usually, low <inline-formula><mml:math id="M369" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> and <inline-formula><mml:math id="M370" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> values can be interpreted as a sign of wetter and
colder conditions (Gascoyne, 1992; Genty et al., 2006; Couchoud et al., 2009). However, on a global scale, the 127–123 <inline-formula><mml:math id="M371" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> period is a time of
the highest sea level and warm temperature conditions (Goelzer et al., 2016). At that time, other European records show high sensitivity to changes in
the amount of precipitation. For example, the <inline-formula><mml:math id="M372" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> record of a stalagmite from Bourgeois–Delaunay Cave (Couchoud et al., 2009) shows
millennial variability with amplitudes lower than 1 ‰ (Fig. 6). The <inline-formula><mml:math id="M373" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> changes are reiterated by <inline-formula><mml:math id="M374" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula>
changes (Couchoud et al., 2009). The authors' interpretation considers the influence of the amount of precipitation as the main driver of
<inline-formula><mml:math id="M375" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> changes. Based on the above-mentioned facts recorded in JS9 stalagmite, episodes of low <inline-formula><mml:math id="M376" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> and
<inline-formula><mml:math id="M377" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> values can be interpreted as wet periods with more intensive vegetation.</p>
      <p id="d1e4808">The second part of MIS 5e (122–115 <inline-formula><mml:math id="M378" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>) has ca. 0.3 ‰ higher <inline-formula><mml:math id="M379" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> values and ca. 0.5 ‰ higher
<inline-formula><mml:math id="M380" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> values. Globally, this period is characterised by a systematic deterioration of climatic conditions, a global mean temperature
decrease of approximately 1 <inline-formula><mml:math id="M381" display="inline"><mml:mrow class="unit"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:math></inline-formula> (Goelzer et al., 2016) and a sea level decrease of approximately 30 <inline-formula><mml:math id="M382" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">m</mml:mi></mml:mrow></mml:math></inline-formula> (Grant et al., 2012). The
NGRIP record shows a 6 ‰ decrease, which reflects the changes in thermal conditions (Rasmussen et al., 2014). At the local scale, the
elevated <inline-formula><mml:math id="M383" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value is the response to a decline in vegetation conditions. The increase in the <inline-formula><mml:math id="M384" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value is caused
by aridification. Evaporation overcomes the temperature effect, which should be negative. A similar episode containing high <inline-formula><mml:math id="M385" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> and
<inline-formula><mml:math id="M386" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> values at approximately 119–117 <inline-formula><mml:math id="M387" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> is observed in the records from Baradla Cave and Magurska Cave; these records are
interpreted as indicating an episode of dry continental climate (Demény et al., 2017; Pawlak et al., 2020). Records from Magurska Cave, located in
the Tatra Mountains, and from Baradla Cave, located in Hungary (Fig. 6), are more similar to the JS9 record during the 122–115 <inline-formula><mml:math id="M388" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> period than
during the older 127–123 <inline-formula><mml:math id="M389" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> period, which suggests that the climate of the region becomes more uniform at the end of MIS 5e.</p>
      <p id="d1e4955">During the 108–101 <inline-formula><mml:math id="M390" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> interval, the <inline-formula><mml:math id="M391" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> and <inline-formula><mml:math id="M392" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> values of the JS9 stalagmite were elevated, and their
values were the highest in comparison to the whole recorded period. The mean value of <inline-formula><mml:math id="M393" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> for this interval was ca. 1.3 ‰
higher than the mean <inline-formula><mml:math id="M394" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value for Holocene in the DCS (Fig. 4). Elevated stable isotope values are related to elevated Fe, Mn, P
and Na contents. In the case of the JS9 stalagmite, the interpretation of the 108–101 <inline-formula><mml:math id="M395" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> period as a dry interval is probable and is in
accordance with <inline-formula><mml:math id="M396" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula>, <inline-formula><mml:math id="M397" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> and Mg proxies. Elevated values of geochemical proxies are related to micrite
microfabrics (Fig. 4k). It appears plausible that the presence of micrite fabric (M) is indicative of bio-influenced processes, as micrite layers may
be associated with shifts to more positive C isotope ratios (Kaźmierczak et al., 1996). In a sample from the Nullarbor, <inline-formula><mml:math id="M398" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">13</mml:mn></mml:msup><mml:mi mathvariant="normal">C</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> values
shift from <inline-formula><mml:math id="M399" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>10.5 ‰ to <inline-formula><mml:math id="M400" display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>4.0 ‰ in stromatolitic-like micrite (M) layers. This phenomenon was interpreted as a possible result of
microbial colonisation of the speleothem surface during a dry period (Frisia, 2015). According to all of this information, the 108–101 <inline-formula><mml:math id="M401" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>
interval in the JS9 stalagmite record can be interpreted as a stable dry continental climatic period.</p>
      <p id="d1e5103">In contrast, the global and regional situations at that time are different. After 110 <inline-formula><mml:math id="M402" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>, the decrease in the global ocean level ended. From
108 to 101 <inline-formula><mml:math id="M403" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>, the world ocean level became elevated to 20 <inline-formula><mml:math id="M404" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">m</mml:mi></mml:mrow></mml:math></inline-formula> in comparison to the local minimum at 110 <inline-formula><mml:math id="M405" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> (Grant et al.,
2012), which suggests that the global mean annual temperature increased. This trend is also in accordance with increased insolation at that time
(Berger 1978). However, the global sea level was more unstable with changes reaching 10 <inline-formula><mml:math id="M406" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">m</mml:mi></mml:mrow></mml:math></inline-formula>. Similarly, the NGRIP record has elevated
<inline-formula><mml:math id="M407" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> values with a short decreasing disturbance at 105 <inline-formula><mml:math id="M408" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>, which also reflects climatic instability (Fig. 6; Rasmussen
et al., 2014). The climate instability at 108–101 <inline-formula><mml:math id="M409" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> is also expressed by the <inline-formula><mml:math id="M410" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> record from Magurska Cave (Fig. 6) and by
records from the northern rim of the Alps (Boch et al., 2011). In contrast, the JS9 record expresses specific local stable conditions during the
108–101 <inline-formula><mml:math id="M411" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> period. A possible explanation is that the DCS region was constantly influenced by the continental climate at that time, in
contrast to the northern Tatra Mountains and the Alps. The ca. 2 ‰ instant decrease and the lowest <inline-formula><mml:math id="M412" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> value in the JS9 record
at 94–95 <inline-formula><mml:math id="M413" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> are not connected with any significant change in the other measured proxies. However, this episode is expressed in the NALPS
<inline-formula><mml:math id="M414" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> records (Fig. 6) as a 1 ‰ instant drop and stalagmite growth cessation and is correlated with Greenland Stadial 23
(Mosley et al., 2020).</p>
</sec>
</sec>
<sec id="Ch1.S6" sec-type="conclusions">
  <label>6</label><title>Final conclusions</title>
      <p id="d1e5249">Temperature is the main factor shaping the increasing tendency in <inline-formula><mml:math id="M415" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> values in the JS9 stalagmite in the older part of the record
(143–130 <inline-formula><mml:math id="M416" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>). The instant change to negative values during T II could have been caused by source and continental effects, which
overcame the temperature effect at that time. The observed response is the result of both the increase in the mean annual temperature and the
source/circulation effect, which overcame each other. During T II, the records located in the western Alps and on the northern slopes of the
Tatra Mountains had a positive shift in <inline-formula><mml:math id="M417" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula>. This shows that mountains such as the Carpathian Belt and the Alps were important climatic
barriers at that time. The older MIS 5e period (127–123 <inline-formula><mml:math id="M418" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula>) had a warmer and wetter climate. The response of proxies recorded in the JS9
stalagmite was dominated by the influence of wetter conditions. MIS 5e records from the Tatra Mountains and Baradla Cave located in Hungary (Fig. 6) are
more similar to the JS9 record during the 122–115 <inline-formula><mml:math id="M419" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> period than during the older 127–123 <inline-formula><mml:math id="M420" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> period, which suggests that the
climate of the region became more uniform at the end of MIS 5e. The climate instability at 108–101 <inline-formula><mml:math id="M421" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> is also expressed by the
<inline-formula><mml:math id="M422" display="inline"><mml:mrow><mml:mi mathvariant="italic">δ</mml:mi><mml:mrow class="chem"><mml:msup><mml:mi/><mml:mn mathvariant="normal">18</mml:mn></mml:msup><mml:mi mathvariant="normal">O</mml:mi></mml:mrow></mml:mrow></mml:math></inline-formula> record from Magurska Cave (Fig. 6) and by records from the northern rim of the Alps (Boch et al., 2011). In contrast to the
records from the northern rim of the Alps and Tatra Mountains (Fig. 6), the JS9 record expresses specific local stable and dry environmental
conditions during the 108–101 <inline-formula><mml:math id="M423" display="inline"><mml:mrow class="unit"><mml:mi mathvariant="normal">ka</mml:mi></mml:mrow></mml:math></inline-formula> period. A possible explanation is that the DCS region was constantly influenced by the
continental climate at that time, in contrast with the northern Tatra Mountains and the Alps.</p>
</sec>

      
      </body>
    <back><notes notes-type="dataavailability"><title>Data availability</title>

      <?pagebreak page1062?><p id="d1e5350">All U-series ages used for the age–depth model estimation are presented in Table 1. The estimated age–depth model and the isotopic and trace element record data are available in digital form from Figshare:
<ext-link xlink:href="https://doi.org/10.6084/m9.figshare.13116506.v1" ext-link-type="DOI">10.6084/m9.figshare.13116506.v1</ext-link> (Pawlak, 2020). Other data used for the comparison (Fig. 6) are available at <uri>https://www.ncdc.noaa.gov/data-access/paleoclimatology-data/datasets</uri> (North Greenland Ice Core Project members, 2004) as well as in the supplementary materials of the papers cited in the caption of Fig. 6.</p>
  </notes><notes notes-type="competinginterests"><title>Competing interests</title>

      <p id="d1e5362">The author declares that there is no conflict of interest.</p>
  </notes><ack><title>Acknowledgements</title><p id="d1e5368">This study was supported by the National Science Centre of Poland (grant no. 20 15/19/D/ST10/00571). U-series dating and geochemical analyses were supported by the Plan of Institutional Financing of the Institute of Geology, The Czech Academy of Sciences (grant no. RVO 67985831). This research would not have been possible without a permit and help from the Tatra National Park and Slovak Caves Administration. The author would like to thank the reviewers and his colleagues from the Uranium-Series Laboratory (LUT) of the Institute of Geological Sciences, Polish Academy of Sciences, for their constructive comments on this paper.</p></ack><notes notes-type="financialsupport"><title>Financial support</title>

      <p id="d1e5373">This research has been supported by the National Science Centre of Poland (grant no. 20 15/19/D/ST10/00571) and the Czech
Academy of Sciences (grant no. RVO 67985831).</p>
  </notes><notes notes-type="reviewstatement"><title>Review statement</title>

      <p id="d1e5379">This paper was edited by Marie-France Loutre and reviewed by two anonymous referees.</p>
  </notes><ref-list>
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<abstract-html><p>Speleothems are an important source of palaeoclimatic information about the terrestrial environment. The basic advantages of speleothems are their
high preservation potential, the possibility of precise dating using the uranium-series (U-series) method, and many different proxies, such as stable isotopes, trace
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speleothem records can add new beneficial data about past climate changes in the region. The multiproxy record of the JS9 stalagmite, collected in the
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speleothem JS9 shows instantaneous decreasing episodes during Termination II. This indicates that the Carpathian Belt was an important climatic
barrier at that time.</p></abstract-html>
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